« Previous AbstractCitrus mangshanensis Pollen Confers a Xenia Effect on Linalool Oxide Accumulation in Pummelo Fruit by Enhancing the Expression of a Cytochrome P450 78A7 Gene CitLO1    Next AbstractN-Carbamylglutamate and l-Arginine Promote Intestinal Absorption of Amino Acids by Regulating the mTOR Signaling Pathway and Amino Acid and Peptide Transporters in Suckling Lambs with Intrauterine Growth Restriction »

J Agric Food Chem

Title:		l-Arginine Protects Ovine Intestinal Epithelial Cells from Lipopolysaccharide-Induced Apoptosis through Alleviating Oxidative Stress
Author(s):		Zhang H; Peng A; Yu Y; Guo S; Wang M; Wang H;
Address:		"Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology , Yangzhou University , Yangzhou 225009 , P. R. China. Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China , Yangzhou University , Yangzhou 225009 , P. R. China"
Journal Title:		J Agric Food Chem
Year:		2019
Volume:		20190201
Issue:		6
Page Number:		1683 - 1690
DOI:		10.1021/acs.jafc.8b06739
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"This research aims to explore the effect of l-arginine (Arg) upon lipopolysaccharide (LPS)-induced induction of the oxidative stress as well as subsequent apoptosis within ovine intestinal epithelial cells (IOECs). Through a 16 h incubation, cells were divided into four groups and the medium was replaced with different medium as follows: (1) control (Con), Arg-free Dulbecco's modified Eagle's F12 Ham medium (DMEM); (2) Arg treatment, Arg-free DMEM supplemented with 100 muM Arg; (3) LPS treatment, Arg-free DMEM supplemented with 10 mug/mL LPS; (4) LPS with Arg treatment, Arg-free DMEM supplemented with both 10 mug/mL LPS and 100 muM Arg. After culturing for 24 h in different mediums, some characteristics of cells in the four groups were measured. Addition of Arg increased cell viability induced with LPS compared with the LPS group ( p < 0.05). Arg significantly decreased the release of dehydrogenase (LDH) and the production of malonaldehyde (MDA) ( p < 0.05) within IOECs challenged by the LPS. Compared with the LPS group, cells treated with Arg and Arg + LPS increased ( p < 0.05) mRNA as well as protein expression of glutathione peroxidase 1 (GPx1), catalase (CAT), superoxide dismutase 2 (SOD2), B-cell lymphoma 2 (Bcl2), quinone oxidoreductase 1 (NQO1), heme oxygenase (HO-1), and nuclear factor erythroid 2-related factor 2 (Nrf2). IOEC treatment with Arg reduced significantly ( p < 0.05) apoptosis induced by the LPS (12.58 +/- 0.79%). The results showed that Arg promoted the protein expression of Nrf2, up-regulated expression of the phase II metabolizing enzymes (NQO1 and HO-1), as well as antioxidative enzymes (GPx1, CAT, and SOD2) for alleviating oxidative injury and protected IOECs from LPS-induced apoptosis"
Keywords:		Animals Apoptosis/*drug effects Arginine/*administration & dosage Caspase 8/genetics/metabolism Caspase 9/genetics/metabolism Catalase/genetics/metabolism Cell Survival/drug effects Epithelial Cells/cytology/*drug effects/metabolism Heme Oxygenase-1/genet;
Notes:		"MedlineZhang, Hao Peng, Along Yu, Yin Guo, Shuang Wang, Mengzhi Wang, Hongrong eng 2019/01/29 J Agric Food Chem. 2019 Feb 13; 67(6):1683-1690. doi: 10.1021/acs.jafc.8b06739. Epub 2019 Feb 1"