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Anal Bioanal Chem

Title:		Gas chromatography-mass spectrometry untargeted profiling of non-Hodgkin's lymphoma urinary metabolite markers
Author(s):		Bueno Duarte GH; de Piloto Fernandes AMA; Silva AAR; Zamora-Obando HR; Amaral AG; de Sousa Mesquita A; Schmidt-Filho J; Cordeiro de Lima VC; D'Almeida Costa F; Andrade VP; Porcari AM; Eberlin MN; Simionato AVC;
Address:		"ThoMSon Mass Spectrometry Laboratory, University of Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil. Laboratory of Analysis of Biomolecules Tiselius, University of Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil. Health Sciences Postgraduate Program, Sao Francisco University-USF, Braganca Paulista, SP, 12916-900, Brazil. Department of Hematology, AC Camargo Cancer Center, Sao Paulo, SP, 01525-001, Brazil. Department of Medical Oncology, AC Camargo Cancer Center, Sao Paulo, SP, 01525-001, Brazil. Department of Pathology, AC Camargo Cancer Center, Sao Paulo, SP, 01509-010, Brazil. MackMass Laboratory for Mass Spectrometry school of engineering - PPGEMN, Mackenzie Presbyterian University, Sao Paulo, SP, 01302-907, Brazil. Laboratory of Analysis of Biomolecules Tiselius, University of Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil. avsimionato@unicamp.br. National Institute of Science and Technology in Bioanalytics (INCTBio), Campinas, SP, 13083-970, Brazil. avsimionato@unicamp.br"
Journal Title:		Anal Bioanal Chem
Year:		2020
Volume:		20200908
Issue:		27
Page Number:		7469 - 7480
DOI:		10.1007/s00216-020-02881-5
ISSN/ISBN:		1618-2650 (Electronic) 1618-2642 (Linking)
Abstract:		"Non-Hodgkin's lymphoma (NHL) is a cancer of the lymphatic system where the lymphoid and hematopoietic tissues are infiltrated by malignant neoplasms of B, T, and natural killer lymphocytes. Effective and less invasive methods for NHL screening are urgently needed. Herein, we report an untargeted gas chromatography-mass spectrometry (GC-MS) method to investigate metabolic changes in non-volatile derivatized compounds from urine samples of NHL patients (N = 15) and compare them to healthy controls (N = 34). Uni- and multivariate data analysis showed 18 endogenous metabolites, including amino acids and their metabolites, sugars, small organic acids, and vitamins, as statistically significant for group differentiation. A receiver operating characteristic curve (ROC) generated from a support vector machine (SVM) algorithm-based model achieved 0.998 of predictive accuracy, displaying the potential and relevance of GC-MS-detected urinary non-volatile compounds for predictive purposes. Furthermore, a specific panel of key metabolites was also evaluated, showing similar results. All in all, our results indicate that this robust GC-MS method is an effective screening tool for NHL diagnosis and it is able to highlight different pathways of the disease. Graphical Abstract"
Keywords:		"Adult Aged Biomarkers, Tumor/metabolism/urine Female Gas Chromatography-Mass Spectrometry/methods Humans Lymphoma, Non-Hodgkin/metabolism/*urine Male *Metabolome Metabolomics/methods Middle Aged Biomarkers Gas chromatography-mass spectrometry Metabolomics;"
Notes:		"MedlineBueno Duarte, Gustavo Henrique de Piloto Fernandes, Anna Maria Alves Silva, Alex Aparecido Rosini Zamora-Obando, Hans R Amaral, Alan Goncalves de Sousa Mesquita, Alessandra Schmidt-Filho, Jayr Cordeiro de Lima, Vladmir C D'Almeida Costa, Felipe Andrade, Victor Piana Porcari, Andreia M Eberlin, Marcos Nogueira Simionato, Ana Valeria Colnaghi eng 870359/1997-5/National Council for Scientific and Technological Development/ 2016/07014-5/Sao Paulo Research Foundation/ Germany 2020/09/09 Anal Bioanal Chem. 2020 Nov; 412(27):7469-7480. doi: 10.1007/s00216-020-02881-5. Epub 2020 Sep 8"