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J Sep Sci

Title:		Comprehensive two-dimensional gas chromatographic platforms comparison for exhaled breath metabolites analysis
Author(s):		Zanella D; Henin A; Mascrez S; Stefanuto PH; Franchina FA; Focant JF; Purcaro G;
Address:		"Molecular System, Organic & Biological Analytical Chemistry Group, University of Liege, Liege, Belgium. Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium. Department of Chemistry, Pharmaceutical, and Agricultural Sciences, University of Ferrara, Ferrara, Italy"
Journal Title:		J Sep Sci
Year:		2022
Volume:		20220802
Issue:		18
Page Number:		3542 - 3555
DOI:		10.1002/jssc.202200164
ISSN/ISBN:		1615-9314 (Electronic) 1615-9306 (Print) 1615-9306 (Linking)
Abstract:		"The high potential of exhaled breath for disease diagnosis has been highlighted in numerous studies. However, exhaled breath analysis is suffering from a lack of standardized sampling and analysis procedures, impacting the robustness of inter-laboratory results, and thus hampering proper external validation. The aim of this work was to verify compliance and validate the performance of two different comprehensive two-dimensional gas chromatography coupled to mass spectrometry platforms in different laboratories by monitoring probe metabolites in exhaled breath following the Peppermint Initiative guidelines. An initial assessment of the exhaled breath sampling conditions was performed, selecting the most suitable sampling bag material and volume. Then, a single sampling was performed using Tedlar bags, followed by the trapping of the volatile organic compounds into thermal desorption tubes for the subsequent analysis using two different analytical platforms. The thermal desorption tubes were first analyzed by a (cryogenically modulated) comprehensive two-dimensional gas chromatography system coupled to high-resolution time-of-flight mass spectrometry. The desorption was performed in split mode and the split part was recollected in the same tube and further analyzed by a different (flow modulated) comprehensive two-dimensional gas chromatography system with a parallel detection, specifically using a quadrupole mass spectrometer and a vacuum ultraviolet detector. Both the comprehensive two-dimensional gas chromatography platforms enabled the longitudinal tracking of the peppermint oil metabolites in exhaled breath. The increased sensitivity of comprehensive two-dimensional gas chromatography enabled to successfully monitor over a 6.5 h period a total of 10 target compounds, namely alpha-pinene, camphene, beta-pinene, limonene, cymene, eucalyptol, menthofuran, menthone, isomenthone, and neomenthol"
Keywords:		Bicyclic Monoterpenes Breath Tests/methods Cymenes Eucalyptol/analysis Gas Chromatography-Mass Spectrometry/methods Limonene/analysis Polyethylene Terephthalates *Volatile Organic Compounds/analysis breath analysis comprehensive two-dimensional gas chroma;
Notes:		"MedlineZanella, Delphine Henin, Adele Mascrez, Steven Stefanuto, Pierre-Hugues Franchina, Flavio Antonio Focant, Jean-Francois Purcaro, Giorgia eng Germany 2022/07/20 J Sep Sci. 2022 Sep; 45(18):3542-3555. doi: 10.1002/jssc.202200164. Epub 2022 Aug 2"