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J Microbiol Methods

Title:		Classification of gases around Pseudomonas aeruginosa and Acinetobacter baumannii by infrared spectroscopy
Author(s):		Yamamoto M; Arata S; Fukuchi K; Honda H; Kobayashi H; Inagaki M;
Address:		"Department of Chemistry, College of Arts and Sciences, Showa University, 4562 Kamiyoshida, Fujiyoshida-city, Yamanashi 403-0005, Japan. Electronic address: yama@cas.showa-u.ac.jp. Department of Chemistry, College of Arts and Sciences, Showa University, 4562 Kamiyoshida, Fujiyoshida-city, Yamanashi 403-0005, Japan; Center for Biotechnology, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. Graduate School of Health Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. Department of Chemistry, College of Arts and Sciences, Showa University, 4562 Kamiyoshida, Fujiyoshida-city, Yamanashi 403-0005, Japan"
Journal Title:		J Microbiol Methods
Year:		2022
Volume:		20220425
Issue:
Page Number:		106474 -
DOI:		10.1016/j.mimet.2022.106474
ISSN/ISBN:		1872-8359 (Electronic) 0167-7012 (Linking)
Abstract:		"Gas samples were collected from the air surrounding single and mixed laboratory cultures, and preliminary data on human breath samples were also obtained. The infrared spectra of a variety of gasses were measured at high resolution (0.5 cm(-1)) to obtain information about the infrared absorption bands to be used as indicators. These key bands enable bacterial classification, and the discrimination rates can be improved by observing multiple infrared absorptions. The air around Pseudomonas aeruginosa was distinguished from the other gas samples by the infrared absorptions at wavenumbers of 778.4 cm(-1) and 2213.2 cm(-1). For Acinetobacter baumannii, infrared absorptions at 1215.0 cm(-1) and 2982.3 cm(-1) were used; furthermore, adding those at 4768.7 cm(-1) and 5353.8 cm(-1) was shown to improve identification"
Keywords:		"*Acinetobacter baumannii Anti-Bacterial Agents/therapeutic use Gases Humans Microbial Sensitivity Tests *Pseudomonas Infections/microbiology Pseudomonas aeruginosa Spectrophotometry, Infrared Acinetobacter baumannii Fourier transform infrared spectroscopy;"
Notes:		"MedlineYamamoto, Masato Arata, Satoru Fukuchi, Kunihiko Honda, Hidehiko Kobayashi, Hirokazu Inagaki, Masahiro eng Research Support, Non-U.S. Gov't Netherlands 2022/04/27 J Microbiol Methods. 2022 May; 196:106474. doi: 10.1016/j.mimet.2022.106474. Epub 2022 Apr 25"