| Title: | Molecular characterization of AMP-activated protein kinase alpha2 from herbivorous fish Megalobrama amblycephala and responsiveness to glucose loading and dietary carbohydrate levels |
| Author(s): | Xu C; Liu WB; Zhang DD; Wang KZ; Xia SL; Li XF; |
| Address: | "Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, People's Republic of China. Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, People's Republic of China. Electronic address: xfli@njau.edu.cn" |
| Journal Title: | Comp Biochem Physiol A Mol Integr Physiol |
| DOI: | 10.1016/j.cbpa.2017.03.008 |
| ISSN/ISBN: | 1531-4332 (Electronic) 1095-6433 (Linking) |
| Abstract: | "This study aimed to characterize the full-length cDNA of AMPKalpha2 in Megalobrama amblycephala, and evaluate its potential role in glucose homeostasis and carbohydrate metabolism. The cDNA obtained covered 1942bp with an open reading frame of 1635bp encoding 545 amino acids. Multiple alignments and phylogenetic analysis revealed a high homology (91-100%) among most fish and higher vertebrates. This AMPKalpha2 mRNA predominantly expressed in muscle, liver and brain, while little in gill and intestine. Then, the AMPKalpha2 expressions were determined in the muscle, liver and brain of fish subjected to a glucose load (injected intraperitoneally with 0, 1.67 and 3.34g glucose per kg body weight) and after a 12-week feeding trial (fed two dietary carbohydrate levels: 30% and 43%), respectively. After the glucose load, plasma glycemia peaked at 1h in fish. Thereafter, it decreased significantly to the basal level at 8h. However, AMPKalpha2 expression in muscle, liver and brain all decreased significantly during the first 2h, then returned to the basal value at 24h. Unlikely, tissue AMPKalpha2 expression of fish receiving saline solution increased significantly during the whole sampling period. Additionally, high-carbohydrate diet enhanced its expression in liver and muscle, but not that in brain. These findings indicated that the AMPKalpha2 gene shared a high degree of conservation with that of the other vertebrates. Muscle, liver and brain AMPKalpha2 expressions were highly induced by glucose administration. Furthermore, high dietary carbohydrate modified its expressions in these tissues" |
| Keywords: | "AMP-Activated Protein Kinases/*genetics/metabolism Animals Body Weight Carbohydrate Metabolism/*genetics Cloning, Molecular Cyprinidae/genetics/*metabolism Dietary Carbohydrates/pharmacology Gene Expression Regulation Gills/metabolism Glucose/*metabolism;" |
| Notes: | "MedlineXu, Chao Liu, Wen-Bin Zhang, Ding-Dong Wang, Kai-Zhou Xia, Si-Lei Li, Xiang-Fei eng Research Support, Non-U.S. Gov't 2017/03/21 Comp Biochem Physiol A Mol Integr Physiol. 2017 Jun; 208:24-34. doi: 10.1016/j.cbpa.2017.03.008. Epub 2017 Mar 16" |
