| Title: | "Candidate genes involved in spiroacetal biosynthesis in the oriental fruit fly, Bactrocera dorsalis" |
| Author(s): | Wu ZZ; Zhang H; Qu MQ; Cui Y; Chen MS; Bin SY; Lin JT; |
| Address: | "Guang Zhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China. Department of Entomology, Kansas State University, Manhattan, KS 66506, USA. Guang Zhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China. Electronic address: linjintian@zhku.edu.cn" |
| Journal Title: | Comp Biochem Physiol Part D Genomics Proteomics |
| DOI: | 10.1016/j.cbd.2019.100601 |
| ISSN/ISBN: | 1878-0407 (Electronic) 1744-117X (Linking) |
| Abstract: | "Spiroacetals are widespread in nature as components of volatile semiochemical secretions from many insect species. The general pathway for spiroacetal biosynthesis in Bactrocera sp. is preliminarily established, but many genes involved in this pathway remain to be characterized. By analyzing transcriptomes of the rectal glands (RGs) from immature and mature females of the oriental fruit fly, Bactrocera dorsalis, a set of genes encoding two acetyl-CoA carboxylases (ACCs), two fatty acid synthases (FASs), eight desaturases (DESs), twelve fatty acyl-CoA reductases (FARs), seventy-two cytochrome P450 enzymes (CYPs), and twenty-three odorant binding proteins (OBPs) were identified. We investigated the expression of candidate genes in immature and mature stages based on the RNA-seq data and Real-time quantitative PCR. Expression profiling revealed that some of these genes were primarily expressed in female rectal glands among different tissues, and were up-regulated in mature females. Semi-quantitative RT-PCR assays were also adapted to examine tissue-specific expression of selected candidate genes. Additionally, their putative functions in spiroacetal synthesis and transportation are proposed. Our study provided large-scale sequence information for further functional studies on spiroacetal biosynthetic pathways" |
| Keywords: | "Acetals/*metabolism Animals Biosynthetic Pathways Female Genes, Insect Male Pheromones/*genetics/metabolism Phylogeny Spiro Compounds/metabolism Tephritidae/enzymology/*genetics/metabolism *Transcriptome Bactrocera dorsalis Biosynthesis Spiroacetal Transc;" |
| Notes: | "MedlineWu, Zhong-Zhen Zhang, He Qu, Meng-Qiu Cui, Yang Chen, Ming-Shun Bin, Shu-Ying Lin, Jin-Tian eng Research Support, Non-U.S. Gov't Netherlands 2019/06/17 Comp Biochem Physiol Part D Genomics Proteomics. 2019 Sep; 31:100601. doi: 10.1016/j.cbd.2019.100601. Epub 2019 Jun 8" |
