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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		Neurophysiologic detector-a selective and sensitive tool in high-performance liquid chromatography
Author(s):		Brondz I; Hamdani el H; Doving K;
Address:		"Department of Biochemistry, University of Oslo, P.O. Box 1041, Blindern, 0316 Oslo, Norway. ilia.brondz@biokjemi.uio.no"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2004
Volume:		800
Issue:		1-Feb
Page Number:		41 - 47
DOI:		10.1016/j.jchromb.2003.07.004
ISSN/ISBN:		1570-0232 (Print) 1570-0232 (Linking)
Abstract:		"In the present study neurons from the olfactory system of the fish crucian carp, Carassius carassius L. were used as components in an in-line neurophysiologic detector (NPD) to measure physiological activities following the separation of substances by high-performance liquid chromatography (HPLC). The skin of crucian carp, C. carassius L. contains pheromones that induce an alarm reaction in conspecifics. Extra-cellular recordings were made from neurons situated in the posterior part of the medial region of the olfactory bulb known to mediate this alarm reaction. The nervous activity of these specific neurons in the olfactory bulb of crucian carp was used as an in-line neurophysiologic detector. HPLC was performed with an HP 1100 model equipped with a diode array detector (DAD) and ChemStation software. An adsorbosphere nucleotide-nucleoside 7 microm column was used to separate the substances in the skin extract using artificial pound water (APW) as the mobile phase. UV spectral detection was performed at 214, 254 and 345 nm, and scans (190-400 nm) were collected continuously. This system enabled the selection of peaks in the chromatogram with fish alarm pheromone activity. The neurons in parts of the olfactory system from different aquatic organisms and vertebrates can be used for the detection of species-specific stimuli such as sexual and alarm signals, food odours, and other physiologically significant substances. NPDs clearly offer new and promising options for in-line HPLC as highly selective and sensitive detectors in biological, medical and pharmaceutical research"
Keywords:		"Animals *Biosensing Techniques Calibration Carps/*physiology Chromatography, High Pressure Liquid/*instrumentation Conditioning, Psychological Nasal Cavity/innervation/physiology Olfactory Mucosa/innervation/physiology Olfactory Receptor Neurons/*physiolo;"
Notes:		"MedlineBrondz, Ilia Hamdani, El Hassan Doving, Kjell eng Research Support, Non-U.S. Gov't Netherlands 2003/12/31 J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Feb 5; 800(1-2):41-7. doi: 10.1016/j.jchromb.2003.07.004"