| Title: | Expression Profiles and Biochemical Analysis of Chemosensory Protein 3 from Nilaparvata lugens (Hemiptera: Delphacidae) |
| Author(s): | Waris MI; Younas A; Ameen A; Rasool F; Wang MQ; |
| Address: | "Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. Department of Entomology, Faculty of Agriculture, Gomal University, Dera Ismail Khan, Pakistan. Department of Agronomy, Faculty of Agriculture, Gomal University, Dera Ismail Khan, Pakistan. National Center for Bioinformatics, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. mqwang@mail.hzau.edu.cn" |
| DOI: | 10.1007/s10886-020-01166-6 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "Insects have evolved highly sensitive olfactory sensory systems to detect plant hosts and mates, with plant volatiles playing an important role in informing insect behavior. Chemosensory proteins (CSPs) are thought to play a key role in this process, but in this respect, there is limited information on brown planthopper Nilaparvata lugens, one of the most destructive pests of rice. To expand our understanding of CSP function in N. lugens we explored expression profiles and binding characteristics of NlugCSP3. The ligands with higher binding affinity were also validated by molecular docking and behavioral assays. NlugCSP3 mRNA was expressed at relatively higher levels in antennae and abdomen of 3-day-old unmated macropterous males as well as in antennae of 3-day mated macropterous and brachypterous females. Fluorescence competitive binding assays revealed that 5 out of 25 candidate volatiles are strong binders (Ki < 10 muM). Behavioral assays revealed that nonadecane and 2-tridecanone, which have high binding affinities in fluorescence competition-binding assays, displayed strong attractiveness to N. lugens. Pursuing this further, molecular docking analysis identified key amino acid residues involved in binding volatile compounds. Overall, our data provide a base for further investigation of the potential physiological functions of CSP3 in Nilaparvata lugens, and extend the function of NlugCSP3 in chemoreception of N. lugens" |
| Keywords: | Age Factors Amino Acids/metabolism Animals Arthropod Antennae/metabolism Female *Gene Expression Gene Expression Profiling Hemiptera/chemistry/*genetics/metabolism Insect Proteins/*genetics/metabolism Ligands Male Molecular Docking Simulation Reproduction; |
| Notes: | "MedlineWaris, Muhammad Irfan Younas, Aneela Ameen, Asif Rasool, Fatima Wang, Man-Qun eng 2016YFD0200807/National Basic Research Program of China (973 Program) (CN)/ 2020/03/04 J Chem Ecol. 2020 Apr; 46(4):363-377. doi: 10.1007/s10886-020-01166-6. Epub 2020 Mar 3" |
