| Title: | Chromatography and isolation of the Kav 1.0 pheromone of female Nippostrongylus brasiliensis (Nematoda) |
| ISSN/ISBN: | 0022-3395 (Print) 0022-3395 (Linking) |
| Abstract: | "The Kav 1.0 pheromone from incubation of mouse-adapted females of Nippostrongylus brasiliensis was soluble in organic solvents, as shown by bioassay of males and presence of a peak at Kav 1.0 during gel filtration. Recovery of the biological activity from the females was enhanced when the Kav 1.0 component was purified by organic extraction-gel filtration, C-18 cartridge separation with 50% methanol, C-18 cartridge-gel filtration, and C-18 cartridge-TLC. The activity recovered from organic extraction, TLC, or gel filtration of solutions in which females had been incubated was less than the above combined techniques. Reverse-phase cartridges retained activity of 20,000 female-hr of incubation without loss and enabled storage of the pheromone component for 3 days at 4 C. The HPLC separation of incubate from females revealed an active peak(s) with a 4- to 5-min retention time. This peak(s) was present also in regions containing activity from other purification procedures" |
| Keywords: | "Animals Biological Assay Chromatography, Gel Chromatography, High Pressure Liquid Chromatography, Thin Layer Female Male Nippostrongylus/*analysis Pheromones/*isolation & purification Sex Attractants/analysis/*isolation & purification;" |
| Notes: | "MedlineWard, J B Bone, L W eng AI-16763/AI/NIAID NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1983/04/01 J Parasitol. 1983 Apr; 69(2):302-6" |
