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J Insect Physiol

Title:		Functional characterization of one sex pheromone receptor (AlucOR4) in Apolygus lucorum (Meyer-Dur)
Author(s):		An X; Khashaveh A; Liu D; Xiao Y; Wang Q; Wang S; Geng T; Gu S; Zhang Y;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Institute of Plant and Environment Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China. Langfang Scientific Research Trial Station, Chinese Academy of Agricultural Sciences, Langfang 065000, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: yjzhang@ippcaas.cn"
Journal Title:		J Insect Physiol
Year:		2020
Volume:		20191125
Issue:
Page Number:		103986 -
DOI:		10.1016/j.jinsphys.2019.103986
ISSN/ISBN:		1879-1611 (Electronic) 0022-1910 (Linking)
Abstract:		"Traps baited with female-produced sex pheromones have been very effective in the monitoring and management of mirid bugs in numerous field trials. However, none of the target odorant receptors for sex pheromone components in Apolygus lucorum have been identified. Here, we identified one candidate sex pheromone receptor, AlucOR4, from A. lucorum. Quantitative real-time PCR (qPCR) analysis revealed that AlucOR4 was antennae-enriched and male-biased in adult A. lucorum. Xenopus oocyte expression system assays demonstrated that AlucOR4/AlucOrco was sensitive to two major sex pheromone constituents and exhibited high sensitivity to (E)-2-hexenyl butyrate (E2HB) and lower sensitivity to hexyl butyrate (HB). The expression level of target mRNA was significantly reduced (>80%) in dsAlucOR4-injected bugs after five days. The electroantennogram (EAG) responses of male antennae to E2HB and HB were also reduced significantly (~40%). Our findings suggest that AlucOR4 is essential to sex pheromone perception in A. lucorum"
Keywords:		"Amino Acid Sequence Animals Female Heteroptera/genetics/growth & development/metabolism/*physiology Insect Proteins/chemistry/*genetics/metabolism Male Nymph/genetics/growth & development/metabolism/physiology Receptors, Pheromone/chemistry/*genetics/meta;"
Notes:		"MedlineAn, Xingkui Khashaveh, Adel Liu, Danfeng Xiao, Yong Wang, Qi Wang, Shanning Geng, Ting Gu, Shaohua Zhang, Yongjun eng Research Support, Non-U.S. Gov't England 2019/11/30 J Insect Physiol. 2020 Jan; 120:103986. doi: 10.1016/j.jinsphys.2019.103986. Epub 2019 Nov 25"