| Title: | Heat production and volatile biosynthesis are linked via alternative respiration in Magnolia denudata during floral thermogenesis |
| Author(s): | Wang R; Chen L; Jia Y; Liu L; Sun L; Liu Y; Li Y; |
| Address: | "National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China. Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China. Institute of Botany, Chinese Academy of Sciences, Beijing, China" |
| ISSN/ISBN: | 1664-462X (Print) 1664-462X (Electronic) 1664-462X (Linking) |
| Abstract: | "Floral thermogenesis is coupled with odor emission in known thermogenic plants. It is widely accepted that elevation in floral temperature can help release of volatile organic compounds (VOCs). However, no information is available about whether floral thermogenesis is associated with VOC biosynthesis. Here, we used RNA-Sequencing (RNA-Seq) to draw a gene expression atlas of floral thermogenesis in Magnolia denudata and captured an upregulation of Alternative Oxidase (AOX) during floral thermogenesis. Western blot analyses also suggested upregulation of AOX during floral thermogenesis. Moreover, oxygen consumption analyses revealed increased activity of the AOX respiration pathway during floral thermogenesis. Using HPLC analyses, we further found that increased AOX respiration substantially promoted production of citric acid by 1.35 folds, which provided fundamental metabolite skeletons for biosynthesis of VOCs. RNA-Seq also showed upregulation of genes regulating lignin catabolism, which was in agreement with in situ Raman chemical imaging of lignin. Taken together, our results suggest the central role of AOX by coupling heat production and VOC biosynthesis in floral thermogenesis of M. denudata" |
| Keywords: | alternative respiration cell wall floral thermogenesis high-throughput sequencing volatile organic compounds; |
| Notes: | "PubMed-not-MEDLINEWang, Ruohan Chen, Ling Jia, Yaping Liu, Liya Sun, Liwei Liu, Yujun Li, Yun eng Switzerland 2022/11/01 Front Plant Sci. 2022 Oct 14; 13:955665. doi: 10.3389/fpls.2022.955665. eCollection 2022" |
