| Title: | Evaluating the effects of allelochemical ferulic acid on Microcystis aeruginosa by pulse-amplitude-modulated (PAM) fluorometry and flow cytometry |
| Author(s): | Wang R; Hua M; Yu Y; Zhang M; Xian QM; Yin DQ; |
| Address: | "State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, PR China. Nanjing Institute of Geography and Limnology, Chinese Academy of Science, 210093, Jiangsu, PR China. State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China. Electronic address: yindq@tongji.edu.cn" |
| DOI: | 10.1016/j.chemosphere.2015.12.109 |
| ISSN/ISBN: | 1879-1298 (Electronic) 0045-6535 (Linking) |
| Abstract: | "We investigated the effects of allelochemical ferulic acid (FA) on a series of physiological and biochemical processes of blue-green algae Microcystis aeruginosa, in order to find sensitive diagnostic variables for allelopathic effects. Algal cell density was significantly suppressed by FA (0.31-5.17 mM) only after 48 h exposure. Inhibitions of photosynthetic parameters (F(v)/F(m) and F(v)'/F(m)') occurred more rapidly than cell growth, and the stimulation of non-photochemical quenching was observed as a feed-back mechanisms induced by photosystem II blockage, determining by PAM fluorometry. Inhibitions on esterase activity, membrane potential and integrity, as well as disturbance on cell size, were all detected by flow cytometry with specific fluorescent markers, although exhibiting varied sensitivities. Membrane potential and esterase activity were identified as the most sensitive parameters (with relatively lower EC50 values), and responded more rapidly (significantly inhibited only after 8 h exposure) than photosynthetic parameters and cell growth, thus may be the primary responses of cyanobacteria to FA exposure. The use of PAM fluorometry and flow cytometry for rapid assessment of those sensitive variables may contribute to future mechanistic studies of allolepathic effects on phytoplankton" |
| Keywords: | Coumaric Acids/*pharmacology Esterases/metabolism Flow Cytometry Fluorometry Microcystis/cytology/*drug effects/growth & development/metabolism Pheromones/*pharmacology Photosynthesis/drug effects Photosystem II Protein Complex/drug effects Phytoplankton/; |
| Notes: | "MedlineWang, Rui Hua, Ming Yu, Yang Zhang, Min Xian, Qi-Ming Yin, Da-Qiang eng Research Support, Non-U.S. Gov't England 2016/01/15 Chemosphere. 2016 Mar; 147:264-71. doi: 10.1016/j.chemosphere.2015.12.109. Epub 2016 Jan 14" |
