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Genetics

Title:		Functional specialization of sensory cilia by an RFX transcription factor isoform
Author(s):		Wang J; Schwartz HT; Barr MM;
Address:		"Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA"
Journal Title:		Genetics
Year:		2010
Volume:		20101005
Issue:		4
Page Number:		1295 - 1307
DOI:		10.1534/genetics.110.122879
ISSN/ISBN:		1943-2631 (Electronic) 0016-6731 (Print) 0016-6731 (Linking)
Abstract:		"In animals, RFX transcription factors govern ciliogenesis by binding to an X-box motif in the promoters of ciliogenic genes. In Caenorhabditis elegans, the sole RFX transcription factor (TF) daf-19 null mutant lacks all sensory cilia, fails to express many ciliogenic genes, and is defective in many sensory behaviors, including male mating. The daf-19c isoform is expressed in all ciliated sensory neurons and is necessary and sufficient for activating X-box containing ciliogenesis genes. Here, we describe the daf-19(n4132) mutant that is defective in expression of the sensory polycystic kidney disease (PKD) gene battery and male mating behavior, without affecting expression of ciliogenic genes or ciliogenesis. daf-19(n4132) disrupts expression of a new isoform, daf-19m (for function in male mating). daf-19m is expressed in male-specific PKD and core IL2 neurons via internal promoters and remote enhancer elements located in introns of the daf-19 genomic locus. daf-19m genetically programs the sensory functions of a subset of ciliated neurons, independent of daf-19c. In the male-specific HOB neuron, DAF-19(M) acts downstream of the zinc finger TF EGL-46, indicating that a TF cascade controls the PKD gene battery in this cell-type specific context. We conclude that the RFX TF DAF-19 regulates ciliogenesis via X-box containing ciliogenic genes and controls ciliary specialization by regulating non-X-box containing sensory genes. This study reveals a more extensive role for RFX TFs in generating fully functional cilia"
Keywords:		Animals Caenorhabditis elegans Caenorhabditis elegans Proteins/physiology Cilia/*physiology DNA-Binding Proteins/*physiology Protein Isoforms Regulatory Factor X Transcription Factors Sensory Receptor Cells/*physiology Transcription Factors/*physiology;
Notes:		"MedlineWang, Juan Schwartz, Hillel T Barr, Maureen M eng Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2010/10/07 Genetics. 2010 Dec; 186(4):1295-307. doi: 10.1534/genetics.110.122879. Epub 2010 Oct 5"