| Title: | Expressing an (E)-beta-farnesene synthase in the chloroplast of tobacco affects the preference of green peach aphid and its parasitoid |
| Author(s): | Wang GP; Yu XD; Fan J; Wang CS; Xia LQ; |
| Address: | "College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology in Arid Areas, Yangling, 712100, China. Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, China. School of Life Science and Technology, Nanyang Normal University, Nanyang, 473061, China. Institute of Crop Protection, the Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China" |
| ISSN/ISBN: | 1744-7909 (Electronic) 1672-9072 (Linking) |
| Abstract: | "(E)-beta-Farnesene (EbetaF) synthase catalyses the production of EbetaF, which for many aphids is the main or only component of the alarm pheromone causing the repellence of aphids and also functions as a kairomone for aphids' natural enemies. Many plants possess EbetaF synthase genes and can release EbetaF to repel aphids. In order to effectively recruit the plant-derived EbetaF synthase genes for aphid control, by using chloroplast transit peptide (CTP) of the small subunit of Rubisco (rbcS) from wheat (Triticum aestivum L.), we targeted AabetaFS1, an EbetaF synthase gene from sweet wormwood (Artemisia annua L.), to the chloroplast of tobacco to generate CTP + AabetaFS1 transgenic lines. The CTP + AabetaFS1 transgenic tobacco plants could emit EbetaF at a level up to 19.25 ng/day per g fresh tissues, 4-12 fold higher than the AabetaFS1 transgenic lines without chloroplast targeting. Furthermore, aphid/parasitoid behavioral bioassays demonstrated that the CTP + AabetaFS1 transgenic tobacco showed enhanced repellence to green peach aphid (Myzus persicae) and attracted response of its parasitoid Diaeretiella rapae, thus affecting aphid infestation at two trophic levels. These data suggest that the chloroplast is an ideal subcellular compartment for metabolic engineering of plant-derived EbetaF synthase genes to generate a novel type of transgenic plant emitting an alarm pheromone for aphid control" |
| Keywords: | "Animals Aphids/*physiology Chloroplasts/*enzymology *Gene Expression Regulation, Plant Host-Parasite Interactions Plants, Genetically Modified/enzymology/genetics Pyrophosphatases/*genetics/*metabolism Tobacco/*enzymology/*genetics (E)-beta-farnesene synt;" |
| Notes: | "MedlineWang, Gen-Ping Yu, Xiu-Dao Fan, Jia Wang, Cheng-She Xia, Lan-Qin eng Research Support, Non-U.S. Gov't China (Republic : 1949- ) 2015/02/04 J Integr Plant Biol. 2015 Sep; 57(9):770-82. doi: 10.1111/jipb.12319. Epub 2015 Jan 30" |
