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Plant Cell

Title:		ODORANT1 regulates fragrance biosynthesis in petunia flowers
Author(s):		Verdonk JC; Haring MA; van Tunen AJ; Schuurink RC;
Address:		"Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 SM Amsterdam, The Netherlands"
Journal Title:		Plant Cell
Year:		2005
Volume:		20050401
Issue:		5
Page Number:		1612 - 1624
DOI:		10.1105/tpc.104.028837
ISSN/ISBN:		1040-4651 (Print) 1532-298X (Electronic) 1040-4651 (Linking)
Abstract:		"Floral scent is important to plant reproduction because it attracts pollinators to the sexual organs. Therefore, volatile emission is usually tuned to the foraging activity of the pollinators. In Petunia hybrida, volatile benzenoids determine the floral aroma. Although the pathways for benzenoid biosynthesis have been characterized, the enzymes involved are less well understood. How production and emission are regulated is unknown. By targeted transcriptome analyses, we identified ODORANT1 (ODO1), a member of the R2R3-type MYB family, as a candidate for the regulation of volatile benzenoids in Petunia hybrida cv W115 (Mitchell) flowers. These flowers are only fragrant in the evening and at night. Transcript levels of ODO1 increased before the onset of volatile emission and decreased when volatile emission declined. Downregulation of ODO1 in transgenic P. hybrida Mitchell plants strongly reduced volatile benzenoid levels through decreased synthesis of precursors from the shikimate pathway. The transcript levels of several genes in this pathway were reduced by suppression of ODO1 expression. Moreover, ODO1 could activate the promoter of the 5-enol-pyruvylshikimate-3-phosphate synthase gene. Flower pigmentation, which is furnished from the same shikimate precursors, was not influenced because color and scent biosynthesis occur at different developmental stages. Our studies identify ODO1 as a key regulator of floral scent biosynthesis"
Keywords:		"Amino Acid Sequence Benzene Derivatives/*metabolism Circadian Rhythm/physiology Down-Regulation/physiology Flowers/*metabolism Gene Expression Regulation, Plant/physiology Molecular Sequence Data Petunia/*metabolism Phylogeny Plant Proteins/genetics/isola;"
Notes:		"MedlineVerdonk, Julian C Haring, Michel A van Tunen, Arjen J Schuurink, Robert C eng England 2005/04/05 Plant Cell. 2005 May; 17(5):1612-24. doi: 10.1105/tpc.104.028837. Epub 2005 Apr 1"