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Plasmid

Title:		Mutants of Enterococcus faecalis deficient as recipients in mating with donors carrying pheromone-inducible plasmids
Author(s):		Trotter KM; Dunny GM;
Address:		"Department of Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853"
Journal Title:		Plasmid
Year:		1990
Volume:		24
Issue:		1
Page Number:		57 - 67
DOI:		10.1016/0147-619x(90)90025-8
ISSN/ISBN:		0147-619X (Print) 0147-619X (Linking)
Abstract:		"From Enterococcus faecalis cells containing random chromosomal insertions of Tn916, strains resistant to a lytic phage were selected and tested for conjugal mating ability. The phage-resistant strains all showed decreased recipient ability (Con-) in broth matings with donors carrying pheromone-inducible plasmids. These strains were normal with respect to donor ability in broth matings and recipient ability in filter matings. The data suggest that the mutants are deficient in the binding substance receptor for the pheromone-induced donor aggregation substance. These mutants contained multiple insertions of Tn916, and none of the individual insertions from the mutant strains were capable of generating the phenotype. Analysis of cell envelope lipoteichoic acids and protein revealed changes in both associated with the Con- phenotype"
Keywords:		"Bacteriophages Cell Membrane/metabolism Conjugation, Genetic Enterococcus faecalis/drug effects/*genetics/metabolism Fatty Acids/metabolism Lipopolysaccharides/metabolism Mutation Pheromones/pharmacology *Plasmids/drug effects Teichoic Acids/metabolism Tr;"
Notes:		"MedlineTrotter, K M Dunny, G M eng AI 19310/AI/NIAID NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1990/07/01 Plasmid. 1990 Jul; 24(1):57-67. doi: 10.1016/0147-619x(90)90025-8"