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PLoS One

Title:		Biomolecular changes that occur in the antennal gland of the giant freshwater prawn (Machrobrachium rosenbergii)
Author(s):		Bose U; Kruangkum T; Wang T; Zhao M; Ventura T; Mitu SA; Hodson MP; Shaw PN; Sobhon P; Cummins SF;
Address:		"Genetic, Ecology and Physiology Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, Queensland, Australia. Metabolomics Australia, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland, Australia. Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand. Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, Thailand. S chool of Pharmacy, The University of Queensland, Queensland, Australia. Faculty of Allied Health Sciences, Burapha University, Chonburi, Thailand"
Journal Title:		PLoS One
Year:		2017
Volume:		20170629
Issue:		6
Page Number:		e0177064 -
DOI:		10.1371/journal.pone.0177064
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"In decapod crustaceans, the antennal gland (AnG) is a major primary source of externally secreted biomolecules, and some may act as pheromones that play a major role in aquatic animal communication. In aquatic crustaceans, sex pheromones regulate reproductive behaviours, yet they remain largely unidentified besides the N-acetylglucosamine-1,5-lactone (NAGL) that stimulates male to female attraction. In this study, we used an AnG transcriptome of the female giant freshwater prawn (Macrobrachium rosenbergii) to predict the secretion of 226 proteins, including the most abundantly expressed transcripts encoding the Spaetzle protein, a serine protease inhibitor, and an arthropodial cuticle protein AMP 8.1. A quantitative proteome analysis of the female AnG at intermolt, premolt and postmolt, identified numerous proteins of different abundances, such as the hemocyanin subunit 1 that is most abundant at intermolt. We also show that hemocyanin subunit 1 is present within water surrounding females. Of those metabolites identified, we demonstrate that the NAGL and N-acetylglucosamine (NAG) can bind with high affinity to hemocyanin subunit 1. In summary, this study has revealed components of the female giant freshwater prawn AnG that are released and contribute to further research towards understanding crustacean conspecific signalling"
Keywords:		"Amino Acid Sequence Animals Base Sequence Chromatography, High Pressure Liquid Crustacea/chemistry/*genetics/metabolism Mass Spectrometry Proteome Transcriptome;"
Notes:		"MedlineBose, Utpal Kruangkum, Thanapong Wang, Tianfang Zhao, Min Ventura, Tomer Mitu, Shahida Akter Hodson, Mark P Shaw, Paul N Sobhon, Prasert Cummins, Scott F eng 2017/07/01 PLoS One. 2017 Jun 29; 12(6):e0177064. doi: 10.1371/journal.pone.0177064. eCollection 2017"