| Title: | Modified lactic acid bacteria detect and inhibit multiresistant enterococci |
| Author(s): | Borrero J; Chen Y; Dunny GM; Kaznessis YN; |
| Address: | "daggerDepartment of Chemical Engineering and Materials Science, double daggerDepartment of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, United States" |
| ISSN/ISBN: | 2161-5063 (Electronic) 2161-5063 (Print) 2161-5063 (Linking) |
| Abstract: | "We designed Lactococcus lactis to detect Enterococcus faecalis. Upon detection, L. lactis produce and secrete antienterococcal peptides. The peptides inhibit enterococcal growth and reduce viability of enterococci in the vicinity of L. lactis. The enterococcal sex pheromone cCF10 serves as the signal for detection. Expression vectors derived from pCF10, a cCF10-responsive E. faecalis sex-pheromone conjugative plasmid, were engineered in L. lactis for the detection system. Recombinant host strains were engineered to express genes for three bacteriocins, enterocin A, hiracin JM79 and enterocin P, each with potent antimicrobial activity against E. faecalis. Sensitive detection and specific inhibition occur both in agar and liquid media. The engineered L. lactis also inhibited growth of multidrug-resistant E. faecium strains, when induced by cCF10. The presented vectors and strains can be components of a toolbox for the development of alternative antibiotic technologies targeting enterococci at the site of infection" |
| Keywords: | Anti-Bacterial Agents/metabolism/*pharmacology Bacteriocin Plasmids/*genetics Bacteriocins/genetics/metabolism/*pharmacology Biosensing Techniques/*methods Enterococcus faecalis/*drug effects/*isolation & purification Lactococcus lactis/*genetics/metaboli; |
| Notes: | "MedlineBorrero, Juan Chen, Yuqing Dunny, Gary M Kaznessis, Yiannis N eng R01 GM086865/GM/NIGMS NIH HHS/ R01 GM049530/GM/NIGMS NIH HHS/ GM086865/GM/NIGMS NIH HHS/ GM049530/GM/NIGMS NIH HHS/ R01 GM111358/GM/NIGMS NIH HHS/ Research Support, American Recovery and Reinvestment Act Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2014/06/05 ACS Synth Biol. 2015 Mar 20; 4(3):299-306. doi: 10.1021/sb500090b. Epub 2014 Jun 16" |
