| Title: | Determination and Profiling of Human Skin Odors Using Hair Samples |
| Author(s): | Tavares DS; Mesquita PRR; Salgado VR; Rodrigues FM; Miranda JC; Barral-Netto M; de Andrade JB; Barral A; |
| Address: | "Faculdade de Medicina do Centro Universitario Christus (UNICHRISTUS), Fortaleza, CE 60190-060, Brazil. divast.bio@gmail.com. Instituto Goncalo Moniz (IGM)-Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, BA 40296-710, Brazil. divast.bio@gmail.com. Instituto de Quimica da Universidade Federal da Bahia (UFBA), Salvador, BA 40170-115, Brazil. Programa de Pos-graduacao em Desenvolvimento Regional e Meio Ambiente, Faculdade Maria Milza (FAMAM), Governador Mangabeira, BA 44350-000, Brazil. Faculdade de Medicina Veterinaria da Uniao Metropolitana de Educacao e Cultura (UNIME), Lauro de Freitas, BA 42700-000, Brazil. Instituto Goncalo Moniz (IGM)-Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, BA 40296-710, Brazil. Faculdade de Medicina da Universidade Federal da Bahia (UFBA), Salvador, BA 40026-010, Brazil. Instituto Nacional de Ciencia e Tecnologia de Investigacao em Imunologia (iii-INCT), Sao Paulo, SP 05403-900, Brazil. Instituto Nacional de Ciencia e Tecnologia em Energia e Ambiente (INCT-EA), Salvador, BA 40170-115, Brazil. Instituto Goncalo Moniz (IGM)-Fundacao Oswaldo Cruz (FIOCRUZ), Salvador, BA 40296-710, Brazil. abarral@bahia.fiocruz.br. Faculdade de Medicina da Universidade Federal da Bahia (UFBA), Salvador, BA 40026-010, Brazil. abarral@bahia.fiocruz.br. Instituto Nacional de Ciencia e Tecnologia de Investigacao em Imunologia (iii-INCT), Sao Paulo, SP 05403-900, Brazil. abarral@bahia.fiocruz.br" |
| DOI: | 10.3390/molecules24162964 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Background. There is no gold standard method for human skin odor determination; several techniques can be applied to collect, extract, transfer, and detect human skin odors. However, none of these methods are suitable for field sampling of a large number of individuals. Objective. The present study aimed to develop a simple, fast, non-invasive, and low-cost method for such a purpose. Methods. Considering that hair from legs can act as a retention mesh of volatile organic compounds (VOCs), samples of leg hairs provided by healthy adult males were collected and solid-phase microextraction (SPME), in headspace (HS) mode, coupled to gas chromatography (GC) and mass spectrometry (MS) analysis of the samples was carried out. A pilot test was applied to detect five quality markers that are frequently reported in human skin odors. Then, several steps were performed for method standardization. The method was applied to 36 different individuals (3 sampled under laboratory conditions and 33 under field conditions), aiming to evaluate its applicability in both environments. Findings. A total of 49 VOCs were identified, and 73.5% of these have been reported in previous studies. Main Conclusions. Hair from legs can be considered an efficient tool for human skin odor sampling and a suitable and practical matrix for human skin odor profile determination by using HS-SPME/GC-MS" |
| Keywords: | Analysis of Variance Gas Chromatography-Mass Spectrometry Hair/*chemistry Humans Odorants/*analysis Skin/*metabolism Solid Phase Microextraction Volatile Organic Compounds/analysis/chemistry/isolation & purification Hs-spme/gc-ms human skin odors volatile; |
| Notes: | "MedlineTavares, Diva S Mesquita, Paulo R R Salgado, Vanessa R Rodrigues, Frederico de Medeiros Miranda, Jose Carlos Barral-Netto, Manoel de Andrade, Jailson B Barral, Aldina eng 2582/2013/Fundacao de Amparo a Pesquisa do Estado da Bahia/ Switzerland 2019/08/25 Molecules. 2019 Aug 15; 24(16):2964. doi: 10.3390/molecules24162964" |
