« Previous AbstractDifferential expression profiling of Oxycarenus laetus Kirby (Hemiptera: Lygaeidae) upon exposure to gossypol    Next AbstractMajor phenolic and volatile compounds and their influence on sensorial aspects in stem-contact fermentation winemaking of Primitivo red wines »

Indian J Microbiol

Title:		Gut Metagenomic Profiling of Gossypol Induced Oxycarenus laetus (Hemiptera: Lygaeidae) Reveals Gossypol Tolerating Bacterial Species
Author(s):		Sureshan SC; Mohideen HS; Nair TS;
Address:		"Bioinformatics and Entomoinformatics Lab, Department of Genetic Engineering, School of Bioengineering, SRM Institute of Science & Technology, Kattankulathur, Tamil Nadu 603203 India. GRID: grid.412742.6. ISNI: 0000 0004 0635 5080"
Journal Title:		Indian J Microbiol
Year:		2022
Volume:		20210818
Issue:		1
Page Number:		54 - 60
DOI:		10.1007/s12088-021-00964-0
ISSN/ISBN:		0046-8991 (Print) 0973-7715 (Electronic) 0046-8991 (Linking)
Abstract:		"Oxycarenus laetus is a cotton pest that primarily feeds on seeds that are rich in gossypol. Though gossypol is toxic to general herbivores, O. laetus does not show such complications and instead grows and reproduces well on cotton plants compared to its other hosts. In this study, we have fed O. laetus with natural and induced gossypol-based diets to explore the difference in its gut microbiota. We performed NGS 16S rRNA amplicon sequencing on the Illumina MiSeq platform and analyzed the data using the QIIME2 pipeline supplemented with Greengenes and EZBioCloud reference databases. We also used culture-based methods to identify a few abundant gut bacteria present in O. laetus. Enterococcus faecalis, Wolbachia bourtzisii, Wolbachia pipientis, Corynebacterium glyciniphilum, Staphylococcus sciuri, and Kocuria rosea were some of the major species that formed the core gut microbiome of O. laetus. We have also observed that some species were present only in the sample with the highest concentration of gossypol, signifying that they might have the potential to degrade gossypol. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12088-021-00964-0"
Keywords:		Bioinformatics Cotton pest Gossypol Gut metagenomics Illumina MiSeq Oxycarenus laetus;
Notes:		"PubMed-not-MEDLINESureshan, Shruthi Chalil Mohideen, Habeeb Shaik Nair, Tejas Shaji eng India 2022/01/25 Indian J Microbiol. 2022 Mar; 62(1):54-60. doi: 10.1007/s12088-021-00964-0. Epub 2021 Aug 18"