| Title: | Comparative Analysis of Acanthopanacis Cortex and Periplocae Cortex Using an Electronic Nose and Gas Chromatography-Mass Spectrometry Coupled with Multivariate Statistical Analysis |
| Author(s): | Sun L; Wu J; Wang K; Liang T; Liu Q; Yan J; Yang Y; Qiao K; Ma S; Wang D; |
| Address: | "Xiangyang Public Inspection and Testing Center, Xiangyang 441000, China" |
| DOI: | 10.3390/molecules27248964 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Chinese Herbal Medicines (CHMs) can be identified by experts according to their odors. However, the identification of these medicines is subjective and requires long-term experience. The samples of Acanthopanacis Cortex and Periplocae Cortex used were dried cortexes, which are often confused in the market due to their similar appearance, but their chemical composition and odor are different. The clinical use of the two herbs is different, but the phenomenon of being confused with each other often occurs. Therefore, we used an electronic nose (E-nose) to explore the differences in odor information between the two species for fast and robust discrimination, in order to provide a scientific basis for avoiding confusion and misuse in the process of production, circulation and clinical use. In this study, the odor and volatile components of these two medicinal materials were detected by the E-nose and by gas chromatography-mass spectrometry (GC-MS), respectively. An E-nose combined with pattern analysis methods such as principal component analysis (PCA) and partial least squares (PLS) was used to discriminate the cortex samples. The E-nose was used to determine the odors of the samples and enable rapid differentiation of Acanthopanacis Cortex and Periplocae Cortex. GC-MS was utilized to reveal the differences between the volatile constituents of Acanthopanacis Cortex and Periplocae Cortex. In all, 82 components including 9 co-contained components were extracted by chromatographic peak integration and matching, and 24 constituents could be used as chemical markers to distinguish these two species. The E-nose detection technology is able to discriminate between Acanthopanacis Cortex and Periplocae Cortex, with GC-MS providing support to determine the material basis of the E-nose sensors' response. The proposed method is rapid, simple, eco-friendly and can successfully differentiate these two medicinal materials by their odors. It can be applied to quality control links such as online detection, and also provide reference for the establishment of other rapid detection methods. The further development and utilization of this technology is conducive to the further supervision of the quality of CHMs and the healthy development of the industry" |
| Keywords: | Gas Chromatography-Mass Spectrometry/methods *Electronic Nose Multivariate Analysis Quality Control Odorants/analysis *Volatile Organic Compounds/analysis Acanthopanacis Cortex Chinese Herbal Medicines Periplocae Cortex electronic nose gas chromatography-; |
| Notes: | "MedlineSun, Li Wu, Jing Wang, Kang Liang, Tiantian Liu, Quanhui Yan, Junfeng Yang, Ying Qiao, Ke Ma, Sui Wang, Di eng Grant No. 2020ACA007/Key Research and development program of Science and Technology Major Project of Hubei Province/ Grant No. 20200116/Scientific Research Project of Drug Administration of Hubei Province/ Switzerland 2022/12/24 Molecules. 2022 Dec 16; 27(24):8964. doi: 10.3390/molecules27248964" |
