| Title: | A member of the claudin superfamily influences formation of the front domain in pheromone-responding yeast cells |
| Author(s): | Sukumar M; DeFlorio R; Pai CY; Stone DE; |
| Address: | "Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA" |
| ISSN/ISBN: | 1477-9137 (Electronic) 0021-9533 (Linking) |
| Abstract: | "Cell polarization in response to chemical gradients is important in development and homeostasis across eukaryota. Chemosensing cells orient toward or away from gradient sources by polarizing along a front-rear axis. Using the mating response of budding yeast as a model of chemotropic cell polarization, we found that Dcv1, a member of the claudin superfamily, influences front-rear polarity. Although Dcv1 localized uniformly on the plasma membrane (PM) of vegetative cells, it was confined to the rear of cells responding to pheromone, away from the pheromone receptor. dcv1Delta conferred mislocalization of sensory, polarity and trafficking proteins, as well as PM lipids. These phenotypes correlated with defects in pheromone-gradient tracking and cell fusion. We propose that Dcv1 helps demarcate the mating-specific front domain primarily by restricting PM lipid distribution" |
| Keywords: | *Saccharomyces cerevisiae/metabolism Pheromones/metabolism *Saccharomyces cerevisiae Proteins/metabolism Cell Membrane/metabolism Cell Polarity/physiology Cell polarity Chemotropism Claudin Membrane domain Pheromone Yeast mating; |
| Notes: | "MedlineSukumar, Madhushalini DeFlorio, Reagan Pai, Chih-Yu Stone, David E eng Research Support, Non-U.S. Gov't England 2023/01/06 J Cell Sci. 2023 Jan 15; 136(2):jcs260048. doi: 10.1242/jcs.260048. Epub 2023 Jan 26" |
