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Gene

Title:		In vitro characterization of a (E)-beta-farnesene synthase from Matricaria recutita L. and its up-regulation by methyl jasmonate
Author(s):		Su S; Liu X; Pan G; Hou X; Zhang H; Yuan Y;
Address:		"College of Life Sciences, Anhui Agricultural University, Hefei, China. College of Life Sciences, Anhui Agricultural University, Hefei, China. Electronic address: zhiwuxue239@163.com"
Journal Title:		Gene
Year:		2015
Volume:		20150619
Issue:		1
Page Number:		58 - 64
DOI:		10.1016/j.gene.2015.06.037
ISSN/ISBN:		1879-0038 (Electronic) 0378-1119 (Linking)
Abstract:		"(E)-beta-farnesene is a sesquiterpene semiochemical that is used extensively by both plants and animals for communication. This acyclic olefin is found in the essential oil of chamomile (Matricaria recutita) and was demonstrated that it could attract natural enemies to reduce cabbage aphids in the Chinese cabbage fields. However, little is known regarding the sequence and function of (E)-beta-farnesene synthase in M. recutita. In this study, we reported a new full-length cDNA encoding (E)-beta-farnesene synthase from M. recutita (Mr-betaFS). The cDNA of Mr-betaFS consisted of 2010bp including 1725bp of coding sequence encoding a protein of 574 amino acids with a molecular weight of 67kDa. The deduced amino acid sequence exhibits a considerably higher homology to betaFS from Artemisia annua (about 92% identity) than to betaFSs from other plants (about 20-40% identity). The recombinant enzyme, produced in Escherichia coli, catalyzed the formation of a single product, (E)-beta-farnesene, from farnesyl diphosphate. Real-time quantitative PCR (qRT-PCR) analysis showed that Mr-betaFS expression was highest in leaves and lowest in disk florets. The treatment of M. recutita with methyl jasmonate (MeJA) significantly enhanced the transcriptional level of betaFS gene and the content of (E)-beta-farnesene in M. recutita. The transcriptional level of betaFS gene was approximately 11.5-fold higher than the control sample and the (E)-beta-farnesene emission level ranged from approximately from 0.082 to 0.695mug/g after 24h induction. Our results laid a solid foundation for later improving crop aphid resistance by transgenic technology and provided an important basic data for the regulation of valuable products from M. recutita"
Keywords:		"Acetates/*pharmacology Amino Acid Sequence Cyclopentanes/*pharmacology Electrophoresis, Polyacrylamide Gel Gas Chromatography-Mass Spectrometry Gene Expression Profiling Gene Expression Regulation, Enzymologic/drug effects Gene Expression Regulation, Plan;"
Notes:		"MedlineSu, Shanshan Liu, Xueyan Pan, Guifang Hou, Xiaojuan Zhang, Huimin Yuan, Yi eng Research Support, Non-U.S. Gov't Netherlands 2015/06/23 Gene. 2015 Oct 15; 571(1):58-64. doi: 10.1016/j.gene.2015.06.037. Epub 2015 Jun 19"