| Title: | A New High-Throughput Screening Method to Detect Antimicrobial Volatiles from Metagenomic Clone Libraries |
| Author(s): | Stocker F; Obermeier MM; Resch K; Berg G; Muller Bogota CA; |
| Address: | "Institute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12/I, 8010 Graz, Austria. Austrian Centre of Industrial Biotechnology, Petersgasse 14/V, 8010 Graz, Austria" |
| DOI: | 10.3390/antibiotics9110726 |
| ISSN/ISBN: | 2079-6382 (Print) 2079-6382 (Electronic) 2079-6382 (Linking) |
| Abstract: | "The ever-growing spread of resistance in medicine and agriculture highlights the need to identify new antimicrobials. Microbial volatile organic compounds (VOCs) are one of the most promising groups of chemicals to meet this need. These rarely exploited molecules exhibit antimicrobial activity and their high vapour pressure makes them ideal for application in surface sterilisation, and in particular, in biofumigation. Therefore, we adapted the previously developed Two Clamp VOCs Assay (TCVA) to a new high-throughput screening for the detection of novel antifungal VOCs from metagenomic clone libraries. As a proof of concept, we tested the new high-throughput TCVA (htTCVA) by sourcing a moss metagenomic library against Fusarium culmorum. This led to the identification of five clones that inhibited the growth of mycelium and spores in vitro by up to 8% and 30% and subsequently, to the identification of VOCs that are potentially, and in part responsible for the clones' antifungal activity. For these VOCs, the in vitro effect of the pure compounds was as high as 100%. These results demonstrate the robustness and feasibility of the htTCVA, which provides access to completely new and unexplored biosynthetic pathways and their secondary metabolites" |
| Keywords: | Fusarium spp.Sphagnum antimicrobials functional metagenomics high-throughput screening volatiles; |
| Notes: | "PubMed-not-MEDLINEStocker, Franz Obermeier, Melanie M Resch, Katharina Berg, Gabriele Muller Bogota, Christina A eng 24.081/Osterreichische Forschungsforderungsgesellschaft/ Switzerland 2020/10/28 Antibiotics (Basel). 2020 Oct 22; 9(11):726. doi: 10.3390/antibiotics9110726" |
