| Title: | The disulfide bond pairing of the pheromones Er-1 and Er-2 of the ciliated protozoan Euplotes raikovi |
| Author(s): | Stewart AE; Raffioni S; Chaudhary T; Chait BT; Luporini P; Bradshaw RA; |
| Address: | "Department of Biological Chemistry, College of Medicine, University of California, Irvine 92717" |
| ISSN/ISBN: | 0961-8368 (Print) 1469-896X (Electronic) 0961-8368 (Linking) |
| Abstract: | "The disulfide pairings of the two Euplotes raikovi pheromones Er-1 and Er-2 have been determined by chemical and mass spectrometric analyses. Cystine-linked peptides from thermolytic digestions of the native molecules were purified by reverse-phase high performance liquid chromatography and identified in the known sequences to make the assignments. The same pairing, Cys(I)-Cys(IV), Cys(II)-Cys(VI), and Cys(III)-Cys(V), was found in both pheromones, suggesting that this pattern occurs commonly throughout this family of molecules. This arrangement of disulfides indicates that the three-dimensional structure is defined by three loops, which can vary in size and charge distribution from one pheromone to another" |
| Keywords: | "Amino Acid Sequence Animals Chromatography, High Pressure Liquid Cystine Disulfides/analysis Euplotes/*physiology Mass Spectrometry Membrane Proteins/*chemistry Molecular Sequence Data Peptide Fragments/isolation & purification Pheromones/*chemistry Prote;" |
| Notes: | "MedlineStewart, A E Raffioni, S Chaudhary, T Chait, B T Luporini, P Bradshaw, R A eng DK32465/DK/NIDDK NIH HHS/ GM38274/GM/NIGMS NIH HHS/ RR00862/RR/NCRR NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1992/06/01 Protein Sci. 1992 Jun; 1(6):777-85. doi: 10.1002/pro.5560010609" |
