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Molecules

Title:		Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential
Author(s):		Blazevic I; Dulovic A; Cikes Culic V; Burcul F; Ljubenkov I; Ruscic M; Generalic Mekinic I;
Address:		"Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, 21000 Split, Croatia. blazevic@ktf-split.hr. Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, 21000 Split, Croatia. azra@ktf-split.hr. School of Medicine, University of Split, Soltanska 2, 21000 Split, Croatia. vedrana.cikes.culic@mefst.hr. Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, 21000 Split, Croatia. franko@ktf-split.hr. Department of Chemistry, University of Split, Faculty of Science, Rudera Boskovica 33, 21000 Split, Croatia. ivica.ljubenkov@pmfst.hr. Department of Biology, University of Split, Faculty of Science, Rudera Boskovica 33, 21000 Split, Croatia. mrus@pmfst.hr. Department of Food Technology and Biotechnology, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, 21000 Split, Croatia. gene@ktf-split.hr"
Journal Title:		Molecules
Year:		2019
Volume:		20190219
Issue:		4
Page Number:		-
DOI:		10.3390/molecules24040741
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"Bunias erucago belongs to the Brassicaceae family, which represents a forgotten crop of the Euro-Mediterranean area. The aim of the present study was to determine the glucosinolate profile in different plant parts and biological properties (antioxidant, anticholinesterase, and cytotoxic activities) of the isolates containing glucosinolate breakdown products. The chemical profiles were determined by using HPLC-PDA-MS/MS of desulfoglucosinolates and GC-MS of glucosinolate degradation products. The analysis of B. erucago showed the presence of seven glucosinolates: gluconapin (1), glucoraphasatin (2), glucoraphenin (3), glucoerucin (4), glucoraphanin (5), glucotropaeolin (6), and glucosinalbin (7). The total glucosinolate content ranged from 7.0 to 14.6 micromol/g of dry weight, with the major glucosinolate glucosinalbin in all parts. The antioxidant activity of all volatile isolates was not notable. At a tested concentration of 227 mug/mL, flower hydro-distillate (FH) showed good AChE inhibition, i.e., 40.9%, while root hydro-distillate (RH) had good activity against BChE, i.e., 54.3%. FH showed the best activity against both tested human bladder cancer cell lines, i.e., against T24 after 72 h, which have IC(50) of 16.0 mug/mL, and against TCCSUP after 48 h with IC(50) of 7.8 mug/mL, and can be considered as highly active. On the other hand, RH showed weak activity against tested cancer cells"
Keywords:		"Antineoplastic Agents/chemistry/pharmacology Antioxidants/chemistry/pharmacology Brassicaceae/*chemistry Cell Line, Tumor Cell Proliferation/drug effects Cholinergic Antagonists/chemistry/pharmacology Chromatography, High Pressure Liquid Gas Chromatograph;"
Notes:		"MedlineBlazevic, Ivica Dulovic, Azra Cikes Culic, Vedrana Burcul, Franko Ljubenkov, Ivica Ruscic, Mirko Generalic Mekinic, Ivana eng IP-2016-06-1316/Hrvatska Zaklada za Znanost/ Switzerland 2019/02/23 Molecules. 2019 Feb 19; 24(4):741. doi: 10.3390/molecules24040741"