| Title: | "Gas chromatographic detection of D-(-)-2,3-butanediol and butyric acid produced by sporeformers in cream-style corn and canned beef noodle soup: collaborative study" |
| Author(s): | Schafer ML; Peeler JT; Bradshaw JG; Hamilton CH; Carver RB; |
| ISSN/ISBN: | 0004-5756 (Print) 0004-5756 (Linking) |
| Abstract: | "A gas chromatographic method that identifies sporeformers as the cause of spoilage in swollen cans of low-acid foods was collaboratively studied in 2 stages. Two organic compounds produced by sporeformers, D-(-)-2,3-butanediol and butyric acid, are measured in the upper phase after centrifugation of the liquid portion of the can contents. Each sample is assayed on 2 packed columns designed for the assay of aqueous solutions of volatile fatty acids, using flame ionization detectors. For study 1, 16 duplicate inoculated cans of cream-style corn and beef noodle soup were sent to 9 collaborators. For study 2, 7 collaborators received 11 duplicate inoculated cans of the 2 foods. Duplicate uninoculated cans of each food served as negative controls. The inocula were 6 sporeforming organisms (4 Clostridium and 2 gas-forming Bacillus species) and 2 nonsporeformers. After the deletion of marginal samples, the percentages of correctly identified sporeformers and nonsporeformers in beef noodle soup were 83 (110/132) and 90 (54/60), respectively; corresponding percentages for cream-style corn were 80 (98/123) and 100 (35/35). The method has been adopted official first action" |
| Keywords: | "Animals Bacteria/*metabolism Bacterial Physiological Phenomena Butylene Glycols/*analysis Butyrates/*analysis Butyric Acid Cattle Chromatography, Gas *Food Microbiology *Food Preservation Meat/*analysis Spores, Bacterial/metabolism Zea mays/*analysis;" |
| Notes: | "MedlineSchafer, M L Peeler, J T Bradshaw, J G Hamilton, C H Carver, R B eng 1985/07/01 J Assoc Off Anal Chem. 1985 Jul-Aug; 68(4):626-31" |
