| Title: | "Essential oils and crude extracts from Chrysanthemum trifurcatum leaves, stems and roots: chemical composition and antibacterial activity" |
| Author(s): | Sassi AB; Skhiri FH; Chraief I; Bourgougnon N; Hammami M; Aouni M; |
| Address: | "Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Faculte de Pharmacie" |
| ISSN/ISBN: | 1347-3352 (Electronic) 1345-8957 (Linking) |
| Abstract: | "The essential oils from the leaves, stems and roots of Chrysanthemum trifurcatum (Desf.) Batt. and Trab. var. macrocephalum (viv.) were obtained by hydrodistillation and their chemical compositions were analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS), in order to get insight into similarities and differences as to their active composition. A total of fifty compounds were identified, constituting 97.84%, 99.02% and 98.20% of total oil composition of the leaves, stems and roots, respectively. Monoterpene hydrocarbons were shown to be the main group of constituents of the leaves and stems parts in the ratio of 67.88% and 51.29%, respectively. But, the major group in the roots oil was found to be sesquiterpene hydrocarbons (70.30%). The main compounds in leaves oil were limonene (26.83%), gamma-terpinene (19.68%), alpha-pinene (9.7%) and alpha-terpenyl acetate (7.16%). The stems oil, contains mainly limonene (32.91%), 4-terpenyl acetate (16.33%) and gamma-terpinene (5.93%), whereas the main compounds in roots oil were alpha-calacorene (25.98%), alpha-cedrene (16.55%), beta-bourbobene (14.91%), elemol (7.45%) and 2-hexenal (6.88%). The crude organic extracts of leaves, stems and roots, obtained by maceration with solvents of increasing polarity: petroleum ether, ethyl acetate and methanol, contained tannins, flavonoids and alkaloids. Meanwhile, essential oils and organic extracts were tested for antibacterial activities against eight Gram-positive and Gram-negative strains, using a microdilution method. The oil and methanolic extact from C. trifurcatum leaves showed a great potential of antibacterial effect against Bacillus subtilis and Staphylococcus epidermidis, with an IC50 range of 31.25-62.5 microg/ml" |
| Keywords: | "Chrysanthemum/*chemistry Drug Resistance, Bacterial Gram-Negative Bacteria/*drug effects Gram-Positive Bacteria/*drug effects Microbial Sensitivity Tests/methods Oils, Volatile/chemistry/*isolation & purification/*pharmacology Plant Extracts/chemistry/*is;" |
| Notes: | "MedlineSassi, Ahlem Ben Skhiri, Fethia Harzallah Chraief, Imed Bourgougnon, Nathalie Hammami, Mohamed Aouni, Mahjoub eng Japan 2014/06/03 J Oleo Sci. 2014; 63(6):607-17. doi: 10.5650/jos.ess13228" |
