| Title: | Assessment of urinary volatile compounds and proteins in the female goat Capra hircus: A pilot study to reveal potential indicators of oestrus |
| Author(s): | Sankarganesh D; Ramachandran R; Suriyakalaa U; Ramesh Saravanakumar V; Archunan G; Akbarsha MA; Achiraman S; |
| Address: | "Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, India. Department of Biotechnology, School of Bio and Chemical Engineering, Kalasalingam Academy of Research and Education, Krishnankoil, India. Department of Microbial Biotechnology, Bharathiar University, Coimbatore, India. Department of Livestock Production and Management, Veterinary College and Research Institute, Namakkal, India. Pheromone Technology Laboratory, Department of Animal Science, Bharathidasan University, Tiruchirappalli, India. National College (Autonomous), Tiruchirappalli, India" |
| ISSN/ISBN: | 1439-0531 (Electronic) 0936-6768 (Linking) |
| Abstract: | "Urine samples of female goats in pro-oestrus, oestrus and post-oestrus phases were analysed for finding oestrus-specific volatile compounds using gas chromatograph-mass spectrometry (GC-MS), and proteins using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). Fourteen urinary volatile were identified covering all three phases among which four compounds, 1-Tetradecanol, n-Pentadecanol, 3-Methylene tridecane and 2-Ethyl-1-dodecene, were unique to oestrus. Also, oestrus urine contained a 25 kDa protein, which was totally absent in pro-oestrus urine, and less-expressed in post-oestrus urine. This protein revealed to be complement C3 fragment. This pilot study, for the first time, reveals the difference in urinary volatile compounds and proteins in the female goat during the different phases of oestrous cycle. The four unique volatile compounds and a 25 kDa protein that appeared as oestrus-specific in this study warrant further investigation to consider them as urinary biomarkers of oestrus in goats" |
| Keywords: | "Amino Acid Sequence Animals Biomarkers/urine Electrophoresis, Polyacrylamide Gel Estrus/*urine Female Goats/*urine Pilot Projects Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Volatile Organic Compounds/*urine biomarkers oestrus detectio;" |
| Notes: | "MedlineSankarganesh, D Ramachandran, R Suriyakalaa, U Ramesh Saravanakumar, V Archunan, G Akbarsha, M A Achiraman, S eng SR/FT/LS-147/Department of Science and Technology, Ministry of Science and Technology/ 2008/Department of Science and Technology, Ministry of Science and Technology/ Germany 2019/01/20 Reprod Domest Anim. 2019 Mar; 54(3):646-651. doi: 10.1111/rda.13407. Epub 2019 Feb 13" |
