| Title: | Human skin volatiles: Passive sampling and GC?ª+x?ª+GC-ToFMS analysis as a tool to investigate the skin microbiome and interactions with anthropophilic mosquito disease vectors |
| Author(s): | Roodt AP; Naude Y; Stoltz A; Rohwer E; |
| Address: | "Department of Chemistry, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa. Department of Chemistry, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa. Electronic address: yvette.naude@up.ac.za. Division of Infectious Diseases, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa" |
| Journal Title: | J Chromatogr B Analyt Technol Biomed Life Sci |
| DOI: | 10.1016/j.jchromb.2018.09.002 |
| ISSN/ISBN: | 1873-376X (Electronic) 1570-0232 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) emanating from the surfaces of human skin are of great interest to researchers in medical and forensic fields, as well as to biologists studying the ecology of blood-feeding insect vectors of human disease. Research involving the comparison of relative abundances of VOCs emanating from human skin is currently limited by the methodology used for sample collection and pre-concentration. The use of in-house developed silicone rubber (polydimethylsiloxane (PDMS)) passive sampling devices constructed in the form of bracelets and anklets was explored to address this need. The easy-to-use samplers were employed as non-invasive passive sampling devices for the non-targeted collection and concentration of volatile human skin emissions prior to thermal desorption thereof coupled with comprehensive gas chromatographic time-of-flight mass spectrometric (GC?ª+x?ª+GC-TOFMS) analysis. Compounds collected were from a wide range of compound classes. Several compounds, notably cyclic ketones, identified have not been previously reported in skin volatile literature. Comparison of normalized unique mass peak area signals has revealed relative quantitative differences and similarities between the samples collected from two individuals' wrists and as well as between an individual's wrist and ankle. The sampling method was evaluated based on its ability to provide many candidate compounds for potential biomarker discovery. The results show the ability of the new sampling method for augmenting the current knowledge on human skin volatile emissions. The samplers are both easy to use and economical. Applications explored include the study of the complex relationships between the human skin microbiome and the attractiveness of individuals to anthropophilic blood host seeking mosquitoes" |
| Keywords: | Adult Gas Chromatography-Mass Spectrometry/*methods Humans Male Microbiota/*physiology Mosquito Vectors/*physiology Skin/*chemistry *Skin Physiological Phenomena Volatile Organic Compounds/*analysis Young Adult GCG__xG__GC-ToFMS Human skin volatiles Mosqu; |
| Notes: | "MedlineRoodt, Alexis P Naude, Yvette Stoltz, Anton Rohwer, Egmont eng Netherlands 2018/09/14 J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Oct 15; 1097-1098:83-93. doi: 10.1016/j.jchromb.2018.09.002. Epub 2018 Sep 3" |
