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Microbiol Res

Title:		Antifungal potential against Sclerotinia sclerotiorum (Lib.) de Bary and plant growth promoting abilities of Bacillus isolates from canola (Brassica napus L.) roots
Author(s):		Ribeiro IDA; Bach E; da Silva Moreira F; Muller AR; Rangel CP; Wilhelm CM; Barth AL; Passaglia LMP;
Address:		"Departamento de Genetica, Instituto de Biociencias, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Goncalves, 9500, Caixa Postal 15.053, 91501-970, Porto Alegre, RS, Brazil. LABRESIS - Laboratorio de Pesquisa em Resistencia Bacteriana, Centro de Pesquisa Experimental, Hospital de Clinicas de Porto Alegre (HCPA), Rua Ramiro Barcelos 2350, Porto Alegre, RS, 90.035-903, Brazil. Departamento de Genetica, Instituto de Biociencias, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Goncalves, 9500, Caixa Postal 15.053, 91501-970, Porto Alegre, RS, Brazil. Electronic address: luciane.passaglia@ufrgs.br"
Journal Title:		Microbiol Res
Year:		2021
Volume:		20210402
Issue:
Page Number:		126754 -
DOI:		10.1016/j.micres.2021.126754
ISSN/ISBN:		1618-0623 (Electronic) 0944-5013 (Linking)
Abstract:		"Endophytic bacteria show important abilities in promoting plant growth and suppressing phytopathogens, being largely explored in agriculture as biofertilizers or biocontrol agents. Bacteria from canola roots were isolated and screened for different plant growth promotion (PGP) traits and biocontrol of Sclerotinia sclerotiorum. Thirty isolates belonging to Bacillus, Paenibacillus, Lysinibacillus, and Microbacterium genera were obtained. Several isolates produced auxin, siderophores, hydrolytic enzymes, fixed nitrogen and solubilized phosphate. Five isolates presented antifungal activity against S. sclerotiorum by the dual culture assay and four of them also inhibited fungal growth by volatile organic compounds production. All antagonistic isolates belonged to the Bacillus genus, and had their genomes sequenced for the search of biosynthetic gene clusters (BGC) related to antimicrobial metabolites. These isolates were identified as Bacillus safensis (3), Bacillus pumilus (1), and Bacillus megaterium (1), using the genomic metrics ANI and dDDH. Most strains showed several common BGCs, including bacteriocin, polyketide synthase (PKS), and non-ribosomal peptide synthetase (NRPS), related to pumilacidin, bacillibactin, bacilysin, and other antimicrobial compounds. Pumilacidin-related mass peaks were detected in acid precipitation extracts through MALDI-TOF analysis. The genomic features demonstrated the potential of these isolates in the suppression of plant pathogens; however, some aspects of plant-bacterial interactions remain to be elucidated"
Keywords:		*Antibiosis Ascomycota/*growth & development/metabolism Bacillus/classification/genetics/isolation & purification/*physiology Bacterial Proteins/genetics/metabolism Brassica napus/growth & development/*microbiology Endophytes/classification/genetics/isola;
Notes:		"MedlineRibeiro, Igor Daniel Alves Bach, Evelise da Silva Moreira, Fernanda Muller, Aline Reis Rangel, Caroline Pinto Wilhelm, Camila Morschbacher Barth, Afonso Luis Passaglia, Luciane Maria Pereira eng Germany 2021/04/14 Microbiol Res. 2021 Jul; 248:126754. doi: 10.1016/j.micres.2021.126754. Epub 2021 Apr 2"