| Title: | 17alpha-Ethinyl estradiol affects anxiety and shoaling behavior in adult male zebra fish (Danio rerio) |
| Author(s): | Reyhanian N; Volkova K; Hallgren S; Bollner T; Olsson PE; Olsen H; Hallstrom IP; |
| Address: | "School of Life Sciences, Sodertorn University, SE-141 86 Huddinge, Sweden" |
| DOI: | 10.1016/j.aquatox.2011.05.009 |
| ISSN/ISBN: | 1879-1514 (Electronic) 0166-445X (Linking) |
| Abstract: | "Ethinyl estradiol is a potent endocrine disrupting compound in fish and ubiquitously present in the aquatic environment. In this study, we exposed adult zebra fish (Danio rerio) males to 0, 5 or 25 ng Ethinyl estradiol/L for 14 days and analyzed the effects on non-reproductive behavior. Effects of treatment of the exposed males was shown by vitellogenin induction, while brain aromatase (CYP 19B) activity was not significantly altered. Both concentrations of Ethinyl estradiol significantly altered the behavior in the Novel tank test, where anxiety is determined as the tendency to stay at the bottom when introduced into an unfamiliar environment. The effects were, however, opposite for the two concentrations. Fish that were exposed to 5 ng/L had longer latency before upswim, fewer transitions to the upper half and shorter total time spent in the upper half compared with control fish, while 25 ng Ethinyl estradiol treatment resulted in shorter latency and more and longer visits to the upper half. The swimming activity of 25, but not 5 ng-exposed fish were slightly but significantly reduced, and these fish tended to spend a lot of time at the surface. We also studied the shoaling behavior as the tendency to leave a shoal of littermates trapped behind a Plexiglas barrier at one end of the test tank. The fish treated with Ethinyl estradiol had significantly longer latency before leaving shoal mates and left the shoal fewer times. Further, the fish exposed to 5 ng/L also spent significantly less time away from shoal than control fish. Fertilization frequency was higher in males exposed to 5 ng/L Ethinyl estradiol when compared with control males, while no spawning was observed after treatment with 25 ng/L. The testes from both treatment groups contained a normal distribution of spermatogenesis stages, and no abnormality in testis morphology could be observed. In conclusion, we have observed effects on two behaviors not related to reproduction in zebra fish males after treatment with Ethinyl estradiol, adding to the ecological consequences of contamination of aquatic environments with estrogenic substances" |
| Keywords: | "Animals Anxiety/*chemically induced Aromatase/genetics/metabolism Behavior, Animal/*drug effects Endocrine Disruptors/*toxicity Ethinyl Estradiol/*toxicity Fertilization/drug effects Male Reproduction/drug effects Swimming Testis/drug effects Vitellogenin;" |
| Notes: | "MedlineReyhanian, Nasim Volkova, Kristina Hallgren, Stefan Bollner, Tomas Olsson, Per-Erik Olsen, Hakan Hallstrom, Inger Porsch eng Research Support, Non-U.S. Gov't Netherlands 2011/06/21 Aquat Toxicol. 2011 Sep; 105(1-2):41-8. doi: 10.1016/j.aquatox.2011.05.009. Epub 2011 May 17" |
