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J Appl Microbiol

Title:		Expanding the Klebsiella pneumoniae volatile metabolome using advanced analytical instrumentation for the detection of novel metabolites
Author(s):		Rees CA; Franchina FA; Nordick KV; Kim PJ; Hill JE;
Address:		"Geisel School of Medicine at Dartmouth, Hanover, NH, USA. Thayer School of Engineering at Dartmouth, Hanover, NH, USA. Dartmouth College, Hanover, NH, USA"
Journal Title:		J Appl Microbiol
Year:		2017
Volume:		20170201
Issue:		3
Page Number:		785 - 795
DOI:		10.1111/jam.13372
ISSN/ISBN:		1365-2672 (Electronic) 1364-5072 (Linking)
Abstract:		"AIMS: The purpose of this study was to identify the volatile molecules produced by the pathogenic Gram-negative bacterium Klebsiella pneumoniae (ATCC 13883) during in vitro growth using comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GCxGC-TOFMS). METHODS AND RESULTS: Klebsiella pneumoniae ATCC 13883 was incubated in lysogeny broth to mid-exponential and stationary growth phases. Headspace volatile molecules from culture supernatants were concentrated using solid-phase microextraction (SPME) and analysed via GCxGC-TOFMS. Ninety-two K. pneumoniae-associated volatile molecules were detected, of which 78 (85%) were detected at both phases of growth and 14 (15%) were detected at either mid-exponential or stationary growth phases. CONCLUSIONS: This study has increased the total number of reported K. pneumoniae-associated volatile molecules from 77 to 150, demonstrating the sensitivity and resolution achieved by employing GCxGC-TOFMS for the analysis of bacterial headspace volatiles. SIGNIFICANCE AND IMPACT OF THE STUDY: This study represents an early-stage comprehensive volatile metabolomic analysis of an opportunistic bacterial pathogen. Characterizing the volatile molecules produced by K. pneumoniae during in vitro growth could provide us with a better understanding of this organisms' metabolism, an area that has not been extensively studied to date"
Keywords:		Gas Chromatography-Mass Spectrometry/methods Klebsiella pneumoniae/growth & development/*metabolism *Metabolome Metabolomics Solid Phase Microextraction Volatile Organic Compounds/analysis/chemistry Klebsiella pneumoniae comprehensive two-dimensional gas;
Notes:		"MedlineRees, C A Franchina, F A Nordick, K V Kim, P J Hill, J E eng England 2016/12/09 J Appl Microbiol. 2017 Mar; 122(3):785-795. doi: 10.1111/jam.13372. Epub 2017 Feb 1"