| Title: | Biological Effects of Glucosinolate Degradation Products from Horseradish: A Horse that Wins the Race |
| Author(s): | Popovic M; Maravic A; Cikes Culic V; Dulovic A; Burcul F; Blazevic I; |
| Address: | "Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, 21000 Split, Croatia. Department of Biology, Faculty of Science, University of Split, Rudera Boskovica 33, 21000 Split, Croatia. Department of Medical Chemistry and Biochemistry, School of Medicine, University of Split, Soltanska 2, 2100 Split, Croatia. Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, 21000 Split, Croatia" |
| ISSN/ISBN: | 2218-273X (Electronic) 2218-273X (Linking) |
| Abstract: | "Horseradish degradation products, mainly isothiocyanates (ITC) and nitriles, along with their precursors glucosinolates, were characterized by GC-MS and UHPLC-MS/MS, respectively. Volatiles from horseradish leaves and roots were isolated using microwave assisted-distillation (MAD), microwave hydrodiffusion and gravity (MHG) and hydrodistillation (HD). Allyl ITC was predominant in the leaves regardless of the isolation method while MAD, MHG, and HD of the roots resulted in different yields of allyl ITC, 2-phenylethyl ITC, and their nitriles. The antimicrobial potential of roots volatiles and their main compounds was assessed against sixteen emerging food spoilage and opportunistic pathogens. The MHG isolate was the most active, inhibiting bacteria at minimal inhibitory concentrations (MICs) from only 3.75 to 30 microg/mL, and fungi at MIC(50) between <0.12 and 0.47 microg/mL. Cytotoxic activity of volatile isolates and their main compounds were tested against two human cancer cell lines using MTT assay after 72 h. The roots volatiles showed best cytotoxic activity (HD; IC(50) = 2.62 mug/mL) against human lung A549 and human bladder T24 cancer cell lines (HD; IC(50) = 0.57 mug/mL). Generally, 2-phenylethyl ITC, which was tested for its antimicrobial and cytotoxic activities along with two other major components allyl ITC and 3-phenylpropanenitrile, showed the best biological activities" |
| Keywords: | Animals Anti-Infective Agents/pharmacology Armoracia/*metabolism Fungi/drug effects Gas Chromatography-Mass Spectrometry/methods Glucosinolates/isolation & purification/*metabolism/*pharmacology Humans Isothiocyanates/chemistry Microbial Sensitivity Tests; |
| Notes: | "MedlinePopovic, Marijana Maravic, Ana Cikes Culic, Vedrana Dulovic, Azra Burcul, Franko Blazevic, Ivica eng IP-2016-06-1316/Hrvatska Zaklada za Znanost/International Research Support, Non-U.S. Gov't Switzerland 2020/02/27 Biomolecules. 2020 Feb 21; 10(2):343. doi: 10.3390/biom10020343" |
