| Title: | "MAT alpha 1 protein, a yeast transcription activator, binds synergistically with a second protein to a set of cell-type-specific genes" |
| DOI: | 10.1016/0092-8674(87)90326-6 |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "We show by electrophoresis mobility shift and by DNAase I footprinting assays that the alpha 1 product of the yeast alpha mating-type locus binds to homologous sequences within the control regions of the three known alpha-specific genes. Binding requires both alpha 1 and a second yeast protein(s) (called PRTF) that is present in all three cell types (a, alpha, and a/alpha); neither protein binds alone. Binding and competition experiments using synthetic oligonucleotides indicate that PRTF binds to only part of the homology found at alpha-specific genes and imply that alpha 1 binds to the remainder. Our results suggest that alpha 1 renders gene expression alpha-specific by creating a binding site for PRTF. Similar experiments lead to the idea that PRTF also plays a role in transcription of a-specific genes. Perhaps a-specificity is achieved through the occlusion of the PRTF binding site by alpha 2, the negative regulator encoded by the alpha mating-type locus" |
| Keywords: | "Base Sequence Binding Sites Binding, Competitive DNA, Fungal/*metabolism DNA, Recombinant Deoxyribonuclease I/metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Fungal Proteins/*metabolism Gene Expression Regulation Mating Factor Pep;" |
| Notes: | "MedlineBender, A Sprague, G F Jr eng GM 00715/GM/NIGMS NIH HHS/ GM 30027/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1987/08/28 Cell. 1987 Aug 28; 50(5):681-91. doi: 10.1016/0092-8674(87)90326-6" |
