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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		Bacterial volatile discovery using solid phase microextraction and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry
Author(s):		Bean HD; Dimandja JM; Hill JE;
Address:		"School of Engineering, University of Vermont, Burlington, VT 05405, USA"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2012
Volume:		20120607
Issue:
Page Number:		41 - 46
DOI:		10.1016/j.jchromb.2012.05.038
ISSN/ISBN:		1873-376X (Electronic) 1570-0232 (Print) 1570-0232 (Linking)
Abstract:		"Bacteria produce unique volatile mixtures that could be used to identify infectious agents to the species, and possibly the strain level. However, due to the immense variety of human pathogens, and the close relatedness of some of these bacteria, the robust identification of the bacterium based on its volatile metabolome is likely to require a large number of volatile compounds for each species. We applied comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOFMS) to the identification of the headspace volatiles of Pseudomonas aeruginosa PA14 grown for 24 h in lysogeny broth. This is the first reported use of GCxGC-TOFMS for the characterization of bacterial headspace volatiles. The analytical purity that is afforded by this chromatographic method facilitated the identification of 28 new P. aeruginosa-derived volatiles, nearly doubling the list of volatiles for this species"
Keywords:		Bacteriological Techniques/*methods Gas Chromatography-Mass Spectrometry/*methods Metabolomics/*methods Organic Chemicals/chemistry/classification/*isolation & purification Pseudomonas aeruginosa/*chemistry/metabolism Solid Phase Microextraction/*methods;
Notes:		"MedlineBean, Heather D Dimandja, Jean-Marie D Hill, Jane E eng P20 GM103496/GM/NIGMS NIH HHS/ P20 RR021905/RR/NCRR NIH HHS/ 8 P20 GM103496-07/GM/NIGMS NIH HHS/ 5P20RR021905-07/RR/NCRR NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Netherlands 2012/06/26 J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Jul 15; 901:41-6. doi: 10.1016/j.jchromb.2012.05.038. Epub 2012 Jun 7"