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J Microbiol Methods

Title:		Detection of volatile metabolites of moulds isolated from a contaminated library
Author(s):		Micheluz A; Manente S; Rovea M; Slanzi D; Varese GC; Ravagnan G; Formenton G;
Address:		"Department of Environmental Sciences, Informatics and Statistic, Ca1' Foscari University, Via Torino 155, 30172 Mestre, Venice, Italy. Electronic address: anna.micheluz@unive.it. Department of Molecular Sciences and Nanosystems, Ca' Foscari University, Via Torino 155, 30123 Mestre, Venice, Italy. Electronic address: manente@unive.it. Dipartimento Regionale Laboratori, Agenzia Regionale per la Prevenzione e Protezione Ambientale del Veneto, Via Lissa 6, 30174 Mestre, Venice, Italy. Electronic address: manuela.rovea@arpa.veneto.it. Department of Environmental Sciences, Informatics and Statistic, Ca1' Foscari University, Via Torino 155, 30172 Mestre, Venice, Italy. Electronic address: debora.slanzi@unive.it. Department of Life Sciences and Systems Biology, University of Turin, Viale Mattioli 25, 10125 Turin, Italy. Electronic address: cristina.varese@unito.it. Department of Molecular Sciences and Nanosystems, Ca' Foscari University, Via Torino 155, 30123 Mestre, Venice, Italy. Electronic address: gprav@unive.it. Dipartimento Regionale Laboratori, Agenzia Regionale per la Prevenzione e Protezione Ambientale del Veneto, Via Lissa 6, 30174 Mestre, Venice, Italy. Electronic address: gianni.formenton@arpa.veneto.it"
Journal Title:		J Microbiol Methods
Year:		2016
Volume:		20160705
Issue:
Page Number:		34 - 41
DOI:		10.1016/j.mimet.2016.07.004
ISSN/ISBN:		1872-8359 (Electronic) 0167-7012 (Linking)
Abstract:		"The principal fungal species isolated from a contaminated library environment were tested for their microbial volatile organic compound (MVOC) production ability. Aspergillus creber, A. penicillioides, Cladosporium cladosporioides, Eurotium chevalieri, E. halophilicum, Penicillium brevicompactum and P. chrysogenum were cultivated on suitable culture media inside sample bottles specifically designed and created for direct MVOC injection to a GC-MS instrument. The fungal emissions were monitored over several weeks to detect changes with the aging of the colonies, monitored also by respirometric tests. A total of 55 different MVOCs were detected and isopropyl alcohol, 3-methyl-1-butanol and 2-butanone were the principal compounds in common between the selected fungal species. Moreover, 2,4-dimethylheptane, 1,4-pentadiene, styrene, ethanol, 2-methyl-1-butanol, acetone, furan and 2-methylfuran were the most detected compounds. For the first time, the MVOC production for particular fungal species was detected. The species A. creber, which belongs to the recently revised group Aspergillus section Versicolores, was characterized by the production of ethanol, furan and 1,4-pentadiene. For the xerophilic fungus E. halophilicum, specific production of acetone, 2-butanone and 1,4-pentadiene was detected, supported also by respirometric data. The results demonstrated the potential use of this method for the detection of fungal contamination phenomena inside Cultural Heritage's preservation environments"
Keywords:		Aspergillus/chemistry/isolation & purification Butanones/analysis Cladosporium/chemistry/isolation & purification Culture Media/chemistry *Environmental Microbiology Fungi/*chemistry/classification Furans/analysis Gas Chromatography-Mass Spectrometry *Lib;
Notes:		"MedlineMicheluz, Anna Manente, Sabrina Rovea, Manuela Slanzi, Debora Varese, Giovanna Cristina Ravagnan, Giampietro Formenton, Gianmaria eng Netherlands 2016/07/10 J Microbiol Methods. 2016 Sep; 128:34-41. doi: 10.1016/j.mimet.2016.07.004. Epub 2016 Jul 5"