| Title: | Genome sequencing progenies of magic mushrooms (Psilocybe subaeruginosa) identifies tetrapolar mating and gene duplications in the psilocybin pathway |
| Author(s): | McTaggart AR; James TY; Slot JC; Barlow C; Fechner N; Shuey LS; Drenth A; |
| Address: | "Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Ecosciences Precinct, Dutton Park, Queensland, Australia. Electronic address: a.mctaggart@uq.edu.au. Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, USA. Department of Plant Pathology, The Ohio State University, Columbus, OH, USA. Entheogenesis Australis, PO Box 2046, Belgrave, Victoria, Australia. Queensland Herbarium, Department of Environment and Science, Brisbane Botanic Gardens Mt Coot-tha, Toowong, Queensland, Australia. Queensland Department of Agriculture and Fisheries, Ecosciences Precinct, Dutton Park, Queensland, Australia. Centre for Horticultural Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Ecosciences Precinct, Dutton Park, Queensland, Australia" |
| DOI: | 10.1016/j.fgb.2022.103769 |
| ISSN/ISBN: | 1096-0937 (Electronic) 1087-1845 (Linking) |
| Abstract: | "Knowledge of breeding systems and genetic diversity is critical to select and combine desired traits that advance new cultivars in agriculture and horticulture. Mushrooms that produce psilocybin, magic mushrooms, may potentially be used in therapeutic and wellness industries, and stand to benefit from genetic improvement. We studied haploid siblings of Psilocybe subaeruginosa to resolve the genetics behind mating compatibility and advance knowledge of breeding. Our results show that mating in P. subaeruginosa is tetrapolar, with compatibility controlled at a homeodomain locus with one copy each of HD1 and HD2, and a pheromone/receptor locus with four homologs of the receptor gene STE3. An additional two pheromone/receptor loci homologous to STE3 do not appear to regulate mating compatibility. Alleles in the psilocybin gene cluster did not vary among the five siblings and were likely homozygous in the parent. Psilocybe subaeruginosa and its relatives have three copies of PsiH genes but their impact on production of psilocybin and its analogues is unknown. Genetic improvement in Psilocybe will require access to genetic diversity from the centre of origin of different species, identification of genes behind traits, and strategies to avoid inbreeding depression" |
| Keywords: | "*Psilocybin *Psilocybe/genetics Gene Duplication Receptors, Pheromone/genetics Pheromones Genes, Mating Type, Fungal Fungal genomics Fungal siblings Genome Sexual reproduction;" |
| Notes: | "MedlineMcTaggart, Alistair R James, Timothy Y Slot, Jason C Barlow, Caine Fechner, Nigel Shuey, Louise S Drenth, Andre eng Research Support, Non-U.S. Gov't 2023/01/02 Fungal Genet Biol. 2023 Mar; 165:103769. doi: 10.1016/j.fgb.2022.103769. Epub 2022 Dec 29" |
