| Title: | New approach methods for assessing indoor air toxicity |
| Author(s): | Mannerstrom M; Dvorakova M; Svobodova L; Rucki M; Kotal F; Vavrous A; Vrbikova V; Kejlova K; Jirova D; Heinonen T; |
| Address: | "The Faculty of Medicine and Health Technology, Arvo Ylpon katu 1, 33014 Tampere University, Finland. National Institute of Public Health, Srobarova 49/48, 100 00 Prague 10, Czech Republic. Medical Faculty of Palacky University, Hnevotinska 976/3, 775 15 Olomouc, Czech Republic" |
| DOI: | 10.1016/j.crtox.2022.100090 |
| ISSN/ISBN: | 2666-027X (Electronic) 2666-027X (Linking) |
| Abstract: | "Indoor air is typically a mixture of many chemicals at low concentrations without any adverse health effects alone, but in mixtures they may cause toxicity and risks to human health. The aim of this study was by using new approach methods to assess the potential toxicity of indoor air condensates. In specific, different in vitro test methods including cyto-and immunotoxicity, skin sensitization and endocrine disruption were applied. In addition to biological effects, the indoor air samples were subjected to targeted analysis of 25 volatile organic compounds (VOCs) and Genapol X-80 (a nonionic emulsifier) suspected to be present in the samples, and to a non-targeted 'total chemical scan' to find out whether the chemical composition of the samples is associated with the biological effects. The results confirm that assessing health risks of indoor air by analysing individual chemicals is not an adequate approach: We were not able to detect the VOCs and Genapol X-80 in the indoor air samples, yet, several types of toxicity, namely, cytotoxicity, immunotoxicity, skin sensitization and endocrine disruption were detected. In the non-targeted total chemical scan of the indoor air samples, a larger number of compounds were found in the cytotoxic samples than in the non-cytotoxic samples supporting the biological findings. If only one biological method would be selected for the screening of indoor air quality, THP-1 macrophage/WST-1 assay would best fit for the purpose as it is sensitive and serves as a good representative for different sub-toxic end points, including immunotoxicity, (skin) sensitization and endocrine disruption" |
| Keywords: | Endocrine disruption Immunological activation Indoor air toxicity Mixture toxicity New approach methods THP-1 macrophage/WST-1 assay; |
| Notes: | "PubMed-not-MEDLINEMannerstrom, Marika Dvorakova, Marketa Svobodova, Lada Rucki, Marian Kotal, Filip Vavrous, Adam Vrbikova, Vera Kejlova, Kristina Jirova, Dagmar Heinonen, Tuula eng Netherlands 2022/10/26 Curr Res Toxicol. 2022 Oct 13; 3:100090. doi: 10.1016/j.crtox.2022.100090. eCollection 2022" |
