| Title: | Differences in diet and biotransformation enzymes of coral reef butterflyfishes between Australia and Hawaii |
| Author(s): | Maldonado A; Nowicki J; Pratchett MS; Schlenk D; |
| Address: | "Department of Environmental Science, University of California, Riverside, CA, 2258 Geology, 900 University Ave., Riverside, CA 92521, USA. Electronic address: amald008@ucr.edu. Department of Biology, Stanford University, Stanford, CA 94305, USA. Electronic address: jnowicki@stanford.edu. ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland 4811, Australia. Electronic address: morgan.pratchett@jcu.edu.au. Department of Environmental Science, University of California, Riverside, CA, 2258 Geology, 900 University Ave., Riverside, CA 92521, USA. Electronic address: dschlenk@ucr.edu" |
| Journal Title: | Comp Biochem Physiol C Toxicol Pharmacol |
| DOI: | 10.1016/j.cbpc.2018.10.004 |
| ISSN/ISBN: | 1532-0456 (Print) 1532-0456 (Linking) |
| Abstract: | "Many reef fishes are capable of feeding on chemically-defended benthic prey, such as soft (alcyonarian) corals; however, little is known about the molecular mechanisms that underpin allelochemical biotransformation and detoxification. Butterflyfishes (Chaetodon: Chaetdontidae) are a useful group for comparatively exploring links between biotransformation enzymes and diet, because they commonly feed on chemically defended prey. Moreover, diets of some species vary among geographic locations. This study compares gene expression, protein and enzymatic activity of key detoxification enzymes (cytochrome P450 (CYP) 2, 3, epoxide hydrolase, glutathione transferase and UDP-glucuronosyltransferase) in livers of four coral-feeding butterflyfish species between Australia and Hawaii, where these fishes differ in diet composition. For C. kleinii, C. auriga, and C. unimaculatus, we found higher CYP2 and CYP3 levels were linked to more allelochemically rich diets in Australia relative to Hawaii. For C. lunulatus from Hawaii CYP2 and CYP3 levels were 1 to 20-fold higher than C. lunulatus from Australia, possibly due to their predominant prey in Hawaii (Porities spp.) being richer in allelochemicals. UGT, GST and epoxide hydrolase varied between species and location and did not correspond to any specific dietary preference or location. Higher levels of CYP2 and CYP3A isozymes in species that feed on allelochemically-rich prey suggest that these biotransformation enzymes may be involved in detoxification of coral dietary allelochemicals in butterflyfishes" |
| Keywords: | "Animals Anthozoa Australia *Biotransformation *Coral Reefs Cytochrome P450 Family 2/*metabolism Cytochrome P450 Family 3/*metabolism Diet/*veterinary Gene Expression Hawaii Inactivation, Metabolic Microsomes, Liver/enzymology Perciformes/*metabolism/physi;" |
| Notes: | "MedlineMaldonado, Aileen Nowicki, Jessica Pratchett, Morgan S Schlenk, Daniel eng Comparative Study 2018/10/28 Comp Biochem Physiol C Toxicol Pharmacol. 2019 Feb; 216:1-9. doi: 10.1016/j.cbpc.2018.10.004. Epub 2018 Oct 24" |
