| Title: | Identification of PLC beta and PKC in pheromone receptor neurons of Antheraea polyphemus |
| Author(s): | Maida R; Redkozubov A; Ziegelberger G; |
| Address: | "Max-Planck-Institut fur Verhaltensphysiologie, Seewiesen, Germany" |
| DOI: | 10.1097/00001756-200006050-00035 |
| ISSN/ISBN: | 0959-4965 (Print) 0959-4965 (Linking) |
| Abstract: | "Two proteins of the IP3 transduction pathway were identified by Western blots in homogenates of isolated pheromone-sensitive sensilla of the silkmoth Antheraea polyphemus. A 110 kDa protein was recognized by an antiserum raised against the Drosophila phospholipase C beta (PLC beta p121) and a 80kDa protein was labelled by an antiserum against a synthetic peptide of a conserved region of protein kinase C (PKC). Incubation of homogenized sensory hairs with the main sex pheromone component, (E,Z) 6-11 hexadecadienyl acetate, resulted in a 6-fold increase in the activity of PKC compared to controls without pheromone. In contrast, incubation with pheromone did not affect the activity of protein kinase A (PKA). Activation of PKC by the membrane permeable dioctanoylglycerol led to excitation of the pheromone-sensitive receptor neurons. These data support the current concept that pheromone perception of moths is mediated by the IP3 transduction pathway" |
| Keywords: | "Acetates/pharmacology Animals Chemoreceptor Cells/*metabolism Diglycerides/pharmacology Enzyme Activation/physiology Isoenzymes/*metabolism Moths/*metabolism Neurons, Afferent/drug effects/enzymology/*metabolism Pheromones/pharmacology Phospholipase C bet;" |
| Notes: | "MedlineMaida, R Redkozubov, A Ziegelberger, G eng England 2000/06/14 Neuroreport. 2000 Jun 5; 11(8):1773-6. doi: 10.1097/00001756-200006050-00035" |
