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Transl Cancer Res

Title:		Analysis of volatile organic compounds released from SW480 colorectal cancer cells and the blood of tumor-bearing mice
Author(s):		Liu D; Ji L; Li M; Li D; Guo L; Nie M; Wang D; Lv Y; Bai Y; Liu M; Wang G; Li Y; Yu P; Li E; Wang C;
Address:		"Department of Anesthesiology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Department of Anesthesiology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China. Department of Anesthesiology, The Affiliated Hospital of Qingdao University, Qingdao 266000, China. Department of Hematology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Department of Anesthesiology, Laishan Branch of Yantai Yuhuangding Hospital, Yantai 264003, China. Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin 150081, China"
Journal Title:		Transl Cancer Res
Year:		2019
Volume:		8
Issue:		8
Page Number:		2736 - 2751
DOI:		10.21037/tcr.2019.10.21
ISSN/ISBN:		2219-6803 (Electronic) 2218-676X (Print) 2218-676X (Linking)
Abstract:		"BACKGROUND: Volatile organic compound (VOC) analysis provides an elegant approach for colorectal cancer screening. An organic compound with a high vapor pressure or volatility can be detected in the headspace of cancer cells or blood samples. Therefore, analyzing VOCs in the blood of rats inoculated with colorectal cancer tissue and in SW480 medium from cultured colorectal cancer cells may provide accurate results. METHODS: After collecting venous blood from rats inoculated with cancer cells at different times, the cancer tissue was removed from the inoculated rats, and the medium was harvested from the cancer cells and cultured in the presence or absence of a chemotherapy drug of intestinal epithelial cells. We used solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) to analyze the headspace of the blood and media to evaluate the VOC profiles. Statistical analysis was conducted using principal component analysis (PCA) and orthogonal partial least-squares analysis (OP-LSDA). RESULTS: The in vivo and in vitro analyses of the colorectal cancer samples revealed a variety of compounds, such as cyclohexanone, 1-hexanol, 2-ethyl-, butylated hydroxytoluene, cyclotrisiloxane, hexamethyl-, pentanoic acid, 2,2,4-trimethyl-3-hydroxy-isobutyl ester and acetone. Butylated hydroxytoluene is unique with regard to its presence during tumor growth and resection; it is also present during tumor cell growth and necrosis. Acetone showed unique trends in the in vivo experimental group. CONCLUSIONS: By analyzing VOC fingerprints related to colorectal cancer (CRC), we found that butylated hydroxytoluene and acetone have unique signatures that may provide the basis for clinical diagnosis and disease assessment"
Keywords:		Acetone biomarkers butylated hydroxytoluene colorectal cancer (CRC) volatile organic compound;
Notes:		"PubMed-not-MEDLINELiu, Desheng Ji, Linlin Li, Mingjuan Li, Dandan Guo, Lei Nie, Maomao Wang, Dongchun Lv, Yang Bai, Yang Liu, Miao Wang, Guiyue Li, Yuhang Yu, Pulin Li, Enyou Wang, Changsong eng China 2019/12/01 Transl Cancer Res. 2019 Dec; 8(8):2736-2751. doi: 10.21037/tcr.2019.10.21"