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J Microbiol

Title:		Expression of sexual genes in Aspergillus fumigatus homogeneous culture produced by vegetative mass mating
Author(s):		Lim JY; Park HM;
Address:		"Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea. Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea. hmpark@cnu.ac.kr"
Journal Title:		J Microbiol
Year:		2019
Volume:		20190511
Issue:		8
Page Number:		688 - 693
DOI:		10.1007/s12275-019-9094-7
ISSN/ISBN:		1976-3794 (Electronic) 1225-8873 (Linking)
Abstract:		"There are presently no studies on the genes for sexual development of Aspergillus fumigatus in situ using mating culture, primarily because of challenging experimental conditions that require a significantly long period of induction and produce developmentally heterogenous culture, harboring very few sexual organs. In order to overcome these challenges, we developed an efficient and convenient procedure called 'vegetative mass mating (VeM)' for study at a molecular level. The VeM method enabled production of a developmentally homogenous A. fumigatus culture, harboring many sexual organs in a plate within a short period of two weeks. Feasibility of the use of VeM for functional study of genes during A. fumigatus sexual development was evaluated by analyzing the transcription pattern of genes involved in pheromone signal transduction and regulation of sexual development. Here, we present for the first time, an in situ expression pattern of sexual genes during the mating process, induced by the VeM method, which will enable and promote the sexual development study of A. fumigatus at the molecular level"
Keywords:		"Aspergillus fumigatus/*genetics Fungal Proteins/*genetics Gene Expression Gene Expression Profiling *Gene Expression Regulation, Fungal *Genes, Mating Type, Fungal Signal Transduction/genetics Aspergillus fumigatus sexual development vegetative mass matin;"
Notes:		"MedlineLim, Joo-Yeon Park, Hee-Moon eng Korea (South) 2019/05/13 J Microbiol. 2019 Aug; 57(8):688-693. doi: 10.1007/s12275-019-9094-7. Epub 2019 May 11"