| Title: | Brassinosteroids Antagonize Jasmonate-Activated Plant Defense Responses through BRI1-EMS-SUPPRESSOR1 (BES1) |
| Author(s): | Liao K; Peng YJ; Yuan LB; Dai YS; Chen QF; Yu LJ; Bai MY; Zhang WQ; Xie LJ; Xiao S; |
| Address: | "State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. School of Life Sciences, Shandong University, Jinan 250110, China. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China xiaoshi3@mail.sysu.edu.cn" |
| ISSN/ISBN: | 1532-2548 (Electronic) 0032-0889 (Print) 0032-0889 (Linking) |
| Abstract: | "Brassinosteroids (BRs) and jasmonates (JAs) regulate plant growth, development, and defense responses, but how these phytohormones mediate the growth-defense tradeoff is unclear. Here, we identified the Arabidopsis (Arabidopsis thaliana) dwarf at early stages1 (dwe1) mutant, which exhibits enhanced expression of defensin genes PLANT DEFENSIN1.2a (PDF1.2a) and PDF1.2b The dwe1 mutant showed increased resistance to herbivory by beet armyworms (Spodoptera exigua) and infection by botrytis (Botrytis cinerea). DWE1 encodes ROTUNDIFOLIA3, a cytochrome P450 protein essential for BR biosynthesis. The JA-inducible transcription of PDF1.2a and PDF1.2b was significantly reduced in the BRASSINOSTEROID INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1 (BES1) gain-of-function mutant bes1- D, which was highly susceptible to S. exigua and B. cinerea BES1 directly targeted the terminator regions of PDF1.2a/PDF1.2b and suppressed their expression. PDF1.2a overexpression diminished the enhanced susceptibility of bes1- D to B. cinerea but did not improve resistance of bes1- D to S. exigua In response to S. exigua herbivory, BES1 inhibited biosynthesis of the JA-induced insect defense-related metabolite indolic glucosinolate by interacting with transcription factors MYB DOMAIN PROTEIN34 (MYB34), MYB51, and MYB122 and suppressing expression of genes encoding CYTOCHROME P450 FAMILY79 SUBFAMILY B POLYPEPTIDE3 (CYP79B3) and UDP-GLUCOSYL TRANSFERASE 74B1 (UGT74B1). Thus, BR contributes to the growth-defense tradeoff by suppressing expression of defensin and glucosinolate biosynthesis genes" |
| Keywords: | Animals Arabidopsis/genetics/*immunology/microbiology/parasitology Arabidopsis Proteins/genetics/*metabolism Botrytis/pathogenicity Brassinosteroids/*biosynthesis/metabolism Cyclopentanes/*metabolism/pharmacology Cytochrome P-450 Enzyme System/genetics/*m; |
| Notes: | "MedlineLiao, Ke Peng, Yu-Jun Yuan, Li-Bing Dai, Yang-Shuo Chen, Qin-Fang Yu, Lu-Jun Bai, Ming-Yi Zhang, Wen-Qing Xie, Li-Juan Xiao, Shi eng Research Support, Non-U.S. Gov't 2019/11/30 Plant Physiol. 2020 Feb; 182(2):1066-1082. doi: 10.1104/pp.19.01220. Epub 2019 Nov 27" |
