| Title: | "Analyzing diurnal and age-related pheromone emission of the olive fruit fly, Bactrocera oleae by sequential SPME-GCMS analysis" |
| Author(s): | Levi-Zada A; Nestel D; Fefer D; Nemni-Lavy E; Deloya-Kahane I; David M; |
| Address: | "Institute of Plant Protection, ARO, Volcani Center, Bet-Dagan 50250, Israel. anatzada@volcani.agri.gov.il" |
| DOI: | 10.1007/s10886-012-0167-x |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "The olive fruit fly, Bactrocera oleae (Diptera: Tephritidae), uses 1,7-dioxaspiro[5.5]undecane ('olean'), produced primarily by females, as a sex pheromone. We used sequential solid phase microextraction-gas chromatography mass spectrometry (SPME-GCMS) analysis to show that female olive flies release about 1000 ng of pheromone at the onset of scotophase for several weeks, while males release about 1/100 as much during the first week after eclosion. The present research demonstrates details of employing SPME-GCMS with the partially known pheromone system of the olive fruit fly as a model for pheromone identification and diurnal release patterns in insects, especially fruit flies. The sequential SPME-GCMS method will readily allow detection and semi-quantification of semiochemicals released by insects in minute amounts throughout the diurnal cycle" |
| Keywords: | Animals Circadian Rhythm Female Gas Chromatography-Mass Spectrometry Male Sex Attractants/*analysis/isolation & purification Solid Phase Microextraction Spiro Compounds/analysis/isolation & purification Stereoisomerism Tephritidae/*metabolism Time Factors; |
| Notes: | "MedlineLevi-Zada, Anat Nestel, David Fefer, Daniela Nemni-Lavy, Esther Deloya-Kahane, Inbal David, Maayan eng Research Support, Non-U.S. Gov't 2012/09/04 J Chem Ecol. 2012 Aug; 38(8):1036-41. doi: 10.1007/s10886-012-0167-x. Epub 2012 Jul 25" |
