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Fish Physiol Biochem

Title:		Cloning and expression of prostaglandin E2 receptor subtype 1 (ep 1 ) in Bostrichthys sinensis
Author(s):		Lai XJ; Hong WS; Liu F; Zhang YT; Chen SX;
Address:		"College of Fisheries, Jimei University, Xiamen, 361021, People's Republic of China"
Journal Title:		Fish Physiol Biochem
Year:		2014
Volume:		20140225
Issue:		4
Page Number:		1281 - 1288
DOI:		10.1007/s10695-014-9923-x
ISSN/ISBN:		1573-5168 (Electronic) 0920-1742 (Linking)
Abstract:		"Our previous studies suggested that prostaglandin E2 (PGE2) is a putative sex pheromone in Chinese black sleeper Bostrichthys sinensis, a fish species that inhabits intertidal zones and mates and spawns inside a muddy burrow. We found immunoreactivities of PGE2 receptor subtypes (Ep1-3) expressed in the olfactory sac, but only Ep1 presented higher density of immunoreactivity in mature fish than that in immature fish in both sexes. To gain a better understanding of the underlying molecular mechanism for the detection of PGE2 in the olfactory system, we cloned an ep 1 cDNA from the adult olfactory sac. The open-reading frame of the ep 1 consisted of 1,134-bp nucleotides that encoded a 378-amino acid-long protein with a seven-transmembrane domain, typical for the G protein-coupled receptors superfamily. Expression of ep 1 mRNA was observed in all tissues examined, with higher levels obtained in the olfactory sacs and testes. The expression of ep 1 mRNA in the olfactory sacs and gonads was significantly higher in both sexes of mature fish than in those of immature ones. Taken together, our results suggested that Ep1, which is highly expressed in the olfactory sacs and gonads of mature fish, is important for the control of reproduction and may be involved in PGE2-initiated spawning behavior in B. sinensis"
Keywords:		"Amino Acid Sequence Analysis of Variance Animals Base Sequence Cloning, Molecular Cluster Analysis Computational Biology DNA Primers/genetics Gonads/metabolism Molecular Sequence Data Olfactory Mucosa/*metabolism Open Reading Frames Perciformes/*genetics/;"
Notes:		"MedlineLai, Xiao Jian Hong, Wan Shu Liu, Fang Zhang, Yu Ting Chen, Shi Xi eng Research Support, Non-U.S. Gov't Netherlands 2014/02/26 Fish Physiol Biochem. 2014 Aug; 40(4):1281-8. doi: 10.1007/s10695-014-9923-x. Epub 2014 Feb 25"