| Title: | GLC determination of bretylium in biological fluids |
| Author(s): | Lai CM; Kamath BL; Carter JE; Erhardt P; Look ZM; Yacobi A; |
| ISSN/ISBN: | 0022-3549 (Print) 0022-3549 (Linking) |
| Abstract: | "Bretylium [(o-bromobenzyl)ethyldimethylamine] is a quaternary ammonium compound used as the tosylate salt for treatment of ventricular fibrillation in humans. A sensitive assay was developed for the determination of low bretylium concentrations in plasma and urine. The internal standards were (p-bromobenzyl)ethyldimethylammonium p-toluenesulfonate and (o-methoxybenzyl)ethyldimethylammonium p-toluenesulfonate. Samples were deproteinized with acetonitrile and extracted with methylene chloride. After the evaporation of the organic phase, the residue was reacted with sodium 2,4,5-trichlorothiophenolate in methanol. This procedure yielded volatile compounds with excellent electron-capture capabilities for the GLC analysis. The assay sensitivity is 5 ng/ml. The extraction recovery of bretylium as determined by a direct radioactivity measurement was 90 and 97% for plasma and urine, respectively. The method is highly reproducible with no significant day-to-day variations. Comparisons of 60 standard plasma samples, 25 standard urine samples, and plasma samples from a dog that received [14C]bretylium showed excellent agreement between the GLC method and direct radioactivity measurement of bretylium" |
| Keywords: | "Animals Bretylium Compounds/*analysis/blood/urine Chromatography, Gas Dogs Humans Male Methods;" |
| Notes: | "MedlineLai, C M Kamath, B L Carter, J E Erhardt, P Look, Z M Yacobi, A eng Comparative Study 1980/06/01 J Pharm Sci. 1980 Jun; 69(6):681-3. doi: 10.1002/jps.2600690618" |
