| Title: | Nutrition Influences Caffeine-Mediated Sleep Loss in Drosophila |
| Author(s): | Keebaugh ES; Park JH; Su C; Yamada R; Ja WW; |
| Address: | "Department of Neuroscience, The Scripps Research Institute, Jupiter, FL. Center on Aging, The Scripps Research Institute, Jupiter, FL. Scripps Graduate Program, The Scripps Research Institute, Jupiter, FL" |
| ISSN/ISBN: | 1550-9109 (Electronic) 0161-8105 (Print) 0161-8105 (Linking) |
| Abstract: | "STUDY OBJECTIVES: Plant-derived caffeine is regarded as a defensive compound produced to prevent herbivory. Caffeine is generally repellent to insects and often used to study the neurological basis for aversive responses in the model insect, Drosophila melanogaster. Caffeine is also studied for its stimulatory properties where sleep or drowsiness is suppressed across a range of species. Since limiting access to food also inhibits fly sleep-an effect known as starvation-induced sleep suppression-we tested whether aversion to caffeinated food results in reduced nutrient intake and assessed how this might influence fly studies on the stimulatory effects of caffeine. METHODS: We measured sleep and total consumption during the first 24 hours of exposure to caffeinated diets containing a range of sucrose concentrations to determine the relative influence of caffeine and nutrient ingestion on sleep. Experiments were replicated using three fly strains. RESULTS: Caffeine reduced total consumption and nighttime sleep, but only at intermediate sucrose concentrations. Although sleep can be modeled by an exponential dose response to nutrient intake, caffeine-mediated sleep loss cannot be explained by absolute caffeine or sucrose ingestion alone. Instead, reduced sleep strongly correlates with changes in total consumption due to caffeine. Other bitter compounds phenocopy the effect of caffeine on sleep and food intake. CONCLUSIONS: Our results suggest that a major effect of dietary caffeine is on fly feeding behavior. Changes in feeding behavior may drive caffeine-mediated sleep loss. Future studies using psychoactive compounds should consider the potential impact of nutrition when investigating effects on sleep" |
| Keywords: | Animals Caffeine/*pharmacology Drosophila melanogaster/*drug effects/*physiology Eating/drug effects/physiology Feeding Behavior/*drug effects/physiology Female Food Deprivation Sleep/*drug effects/physiology Sucrose/administration & dosage/*pharmacology;neuroscience; |
| Notes: | "MedlineKeebaugh, Erin S Park, Jin Hong Su, Chenchen Yamada, Ryuichi Ja, William W eng P40 OD018537/OD/NIH HHS/ R01 AG045036/AG/NIA NIH HHS/ 2017/10/14 Sleep. 2017 Nov 1; 40(11):zsx146. doi: 10.1093/sleep/zsx146" |
