| Title: | Genome-Wide Analysis and Characterization of the Aux/IAA Family Genes Related to Floral Scent Formation in Hedychium coronarium |
| Author(s): | Ke Y; Abbas F; Zhou Y; Yu R; Yue Y; Li X; Yu Y; Fan Y; |
| Address: | "The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. College of Life Sciences, South China Agricultural University, Guangzhou 510642, China. The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. fanyanping@scau.edu.cn. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China. fanyanping@scau.edu.cn" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Auxin plays a key role in different plant growth and development processes, including flower opening and development. The perception and signaling of auxin depend on the cooperative action of various components, among which auxin/indole-3-acetic acid (Aux/IAA) proteins play an imperative role. In a recent study, the entire Aux/IAA gene family was identified and comprehensively analyzed in Hedychium coronarium, a scented species used as an ornamental plant for cut flowers. Phylogenetic analysis showed that the Aux/IAA gene family in H. coronarium is slightly contracted compared to Arabidopsis, with low levels of non-canonical proteins. Sequence analysis of promoters showed numerous cis-regulatory elements related to various phytohormones. HcIAA genes showed distinct expression patterns in different tissues and flower developmental stages, and some HcIAA genes showed significant responses to auxin and ethylene, indicating that Aux/IAAs may play an important role in linking hormone signaling pathways. Based on the expression profiles, HcIAA2, HcIAA4, HcIAA6 and HcIAA12, were selected as candidate genes and HcIAA2 and HcIAA4 were screened for further characterization. Downregulation of HcIAA2 and HcIAA4 by virus-induced gene silencing in H. coronarium flowers modified the total volatile compound content, suggesting that HcIAA2 and HcIAA4 play important roles in H. coronarium floral scent formation. The results presented here will provide insights into the putative roles of HcIAA genes and will assist the elucidation of their precise roles during floral scent formation" |
| Keywords: | "Cell Nucleus/*chemistry/genetics Flowers/chemistry/genetics/growth & development Gene Expression Profiling/*methods Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Indoleacetic Acids/metabolism Multigene Family Odorants/analysi;" |
| Notes: | "MedlineKe, Yanguo Abbas, Farhat Zhou, Yiwei Yu, Rangcai Yue, Yuechong Li, Xinyue Yu, Yunyi Fan, Yanping eng 31770738/National Natural Science Foundation of China/ 31370694/National Natural Science Foundation of China/ 31870690/National Natural Science Foundation of China/ Switzerland 2019/07/04 Int J Mol Sci. 2019 Jul 1; 20(13):3235. doi: 10.3390/ijms20133235" |
