| Title: | Aspergillus luchuensis fatty acid oxygenase ppoC is necessary for 1-octen-3-ol biosynthesis in rice koji |
| Author(s): | Kataoka R; Watanabe T; Yano S; Mizutani O; Yamada O; Kasumi T; Ogihara J; |
| Address: | "Applied Microbiology and Biotechnology Laboratory, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Applied Microbiology and Biotechnology Laboratory, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: watanabe.taisuke@nihon-u.ac.jp. Department of Biochemical Engineering, Graduate School of Sciences and Engineering, Yamagata University, Jonan, Yonezawa, Yamagata 992-8510, Japan. National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan; United Graduate School of Agricultural Science, Kagoshima University, 1-21-24 Korimoto, Kagoshima-shi, Kagoshima 890-8580, Japan; Department of Bioscience and Biotechnology, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan. National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan" |
| DOI: | 10.1016/j.jbiosc.2019.08.010 |
| ISSN/ISBN: | 1347-4421 (Electronic) 1347-4421 (Linking) |
| Abstract: | "Awamori is a distilled spirit produced in Okinawa Prefecture, in southern Japan. Awamori contains the volatile organic compound 1-octen-3-ol, an important flavor component. Here, using solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GCMS), we demonstrate that the black koji mold Aspergillus luchuensis produces 1-octen-3-ol in rice koji. To examine the role of the fatty acid oxygenase genes ppoA and ppoC in 1-octen-3-ol biosynthesis by A. luchuensis, we constructed ppoA and ppoC disruptants, DeltappoA and DeltappoC, respectively, via protoplast-PEG transformation. No clear differences in growth and conidiation were observed between the transformants and the parent strain. Volatile compounds in rice koji prepared using these gene disruptants were analyzed by SPME-GCMS. The amount of 1-octen-3-ol contained in koji produced by the DeltappoA strain was the same as that produced by the parental strain. In contrast, although the DeltappoC strain grew on the rice koji, 1-octen-3-ol was not detected. These results indicate that ppoC is involved in 1-octen-3-ol biosynthesis in A. luchuensis" |
| Keywords: | Aspergillus/genetics/*metabolism Fatty Acids/metabolism Gas Chromatography-Mass Spectrometry Octanols/*metabolism Oryza/microbiology Oxygenases/genetics/*metabolism Solid Phase Microextraction 1-Octen-3-ol Aspergillus luchuensis Awamori Solid-phase microe; |
| Notes: | "MedlineKataoka, Ryousuke Watanabe, Taisuke Yano, Shigekazu Mizutani, Osamu Yamada, Osamu Kasumi, Takafumi Ogihara, Jun eng Japan 2019/10/06 J Biosci Bioeng. 2020 Feb; 129(2):192-198. doi: 10.1016/j.jbiosc.2019.08.010. Epub 2019 Oct 1" |
